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壳聚糖/藻酸盐纳米膜在促进小鼠全层损伤皮肤创面愈合中的作用

, PP. 2109-2114

Keywords: 壳聚糖/藻酸盐,纳米膜,组织工程,细胞支架,创面愈合

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Abstract:

目的评价复合生物材料壳聚糖/藻酸盐纳米膜作为组织工程的细胞支架材料,通过促进小鼠骨髓间充质干细胞(mesenchymalstemcells,MSCs)在其表面的粘附、迁移、增殖及分化,从而加速皮肤创面修复的效果。方法实验动物为BALB/c小鼠,8周龄,于各小鼠背部制备皮肤缺损创面模型。实验先分2组,实验组小鼠背部创面以壳聚糖/藻酸盐纳米膜覆盖,对照组不予该纳米膜覆盖创面,直接粘贴手术贴巾,并分别围绕创缘皮下注射等量绿色荧光蛋白(greenfluorescentprotein,GFP)标记的骨髓间充质干细胞(GFP-MSCs),在术后进行小动物活体成像观察绿色荧光蛋白信号强度,用流式细胞仪检测阳性GFP-MSCs比例,并取创面新生组织行冰冻切片观察GFP-MSCs生长情况;再分别设置空白对照组、纳米膜组、MSCs注射组和纳米膜+MSCs注射组,观察术后各组小鼠创面愈合情况。结果实验组小鼠术后第7天的创面GFP信号强度[(25.97±6.98)×104]明显高于对照组[(6.00±2.84)×104,P<0.05];流式细胞仪检测创面GFP-MSCs水平,术后第5天实验组阳性率[(66.78±7.00)%]明显高于对照组[(33.58±3.62)%,P<0.01],第7天实验组阳性率[(53.03±3.15)%]明显高于对照组[(34.20±7.98)%,P<0.01];术后第14天小鼠新生组织中GFP-MSCs密度实验组[(101.00±15.51)个/视野]明显高于对照组[(25.25±5.07)个/视野,P<0.05];创面愈合观察发现纳米膜+MSCs注射组创面愈合速度快于其他3组(P<0.05)。结论壳聚糖/藻酸盐纳米膜用于皮肤创面,可促进MSCs在体内粘附、生长、分化、迁移,进而促进皮肤组织修复,加速创面愈合,是一种良好的细胞支架材料。

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