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氯丙咪嗪对裸鼠U87胶质瘤模型凋亡作用的体内研究

, PP. 2062-2066

Keywords: 胶质瘤,氯丙咪嗪,TSPO,CytochromeC,体内

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Abstract:

目的研究氯丙咪嗪(chlorimipramine,CIMP)在裸鼠胶质瘤U87细胞肿瘤模型中的作用。方法建立裸鼠胶质瘤U87细胞肿瘤皮下模型,分为肿瘤模型组、氯丙咪嗪组、替莫唑胺组,其中氯丙咪嗪组包括20、40、60mg/kg3个剂量组,每组6只荷瘤鼠。观察肿瘤的生长情况,待肿瘤模型组死亡后处死其余各组剩余小鼠,比较肿瘤体积、生存时间,检测肿瘤组织中TSPO、Cytochrome、Caspase-3蛋白的表达。结果与肿瘤模型组比较,氯丙咪嗪组对肿瘤生长具有抑制趋势,但是差异不显著(P>0.05);氯丙咪嗪组荷瘤鼠生存时间与肿瘤模型组无明显差异(P>0.05),但有延长生存时间的趋势;氯丙咪嗪60mg/kg组中TSPO、CytochromeC表达较肿瘤模型组增高(P<0.05),Caspase-3蛋白表达无统计学差异(P>0.05)。结论氯丙咪嗪虽然有抑制肿瘤生长,延长裸鼠生存时间的趋势,且可能与启动线粒体凋亡途径相关,但其作用效果不明显。

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