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胎盘合体滋养细胞微粒注射对孕鼠肾功能和病理形态损伤的研究

, PP. 2043-2046

Keywords: 合体滋养细胞微粒,肾损害,子痫前期

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Abstract:

目的观察经孕鼠尾静脉注射合体滋养细胞微粒(syncytiotrophoblastmicroparticlesSTBM)后,小鼠肾功能及肾脏病理改变。方法收集孕18dC57小鼠的胎盘,制备小鼠合体滋养细胞微粒,利用电镜观察C57小鼠STBM的形态结构,BCA法测定C57小鼠STBM的蛋白水平;将C57孕鼠分为STBM组、对照组和PBS组,STBM组:自C57小鼠孕8d起,每天以蛋白浓度为0.15mg/mL的STBM(0.2mL)经鼠尾静脉回输至小鼠体内,至孕18d时收集小鼠24h尿液测定尿微量蛋白(M-TP)、尿肌酐(U-Crea)、尿蛋白肌酐比(M-TP/Cr)、尿酸(U-Uric)、24h尿蛋白(24hU-TP),采集全血测定血清尿素氮(BUN)、肌酐(Scr)、胱抑素C(Cys-C),取肾脏标本切片后行HE染色观察肾组织结构变化;对照组:正常孕18d小鼠收集尿液、采集全血、取肾脏进行检测;PBS组:自孕8d起每天经鼠尾静脉注射PBS0.2mL,余处理同STBM组。结果STBM组孕鼠尿液M-TP、M-TP/Cr、24hU-TP明显高于对照组和PBS组(P<0.01),与对照组比较,PBS组U-Uric未见明显变化(P>0.05),而STBM组U-Uric显著降低(P<0.01);STBM组血清BUN、Scr、Cys-C含量显著升高(P<0.01);STBM组肾脏HE染色可见肾小球内皮细胞增生、肿胀、变形,肾小管云雾状变性。结论类子痫前期模型鼠有肾损伤,STBM可能在子痫前期肾损害中起重要作用。

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