全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

降钙素基因相关肽对血管平滑肌细胞的心肌素表达及细胞炎症激活的作用

, PP. 2027-2031

Keywords: 血管平滑肌细胞,降钙素基因相关肽,心肌素,白介素-

Full-Text   Cite this paper   Add to My Lib

Abstract:

目的探讨降钙素基因相关肽(calcitoningene-relatedpeptide,CGRP)对体外培养的血管平滑肌细胞(vascularsmoothmusclecells,VSMCs)心肌素表达及细胞炎症激活的影响。方法取大鼠胸主动脉,以组织块贴壁培养法获得VSMCs,根据不同的处理方案,分为对照组、IL-1β组、CGRP组、心肌素高表达组、心肌素干扰组和CGRP8-37组(CGRP受体拮抗剂组)。Westernblot检测不同组VSMCs中心肌素和IL-6蛋白表达水平。结果以IL-1β处理VSMCs后,细胞培养24、48、72h时IL-6表达水平均高于基线水平(P<0.05),而心肌素水平无显著变化;以高表达心肌素的腺病毒载体转染VSMCs并给予IL-1β处理后,与单纯IL-1β处理比较,各时间点IL-6水平显著下降(P<0.05)。CGRP组中各个时间点VSMCs内心肌素水平较同时间点IL-1β组心肌素水平显著增加[24h:(1.17±0.03)vs(0.25±0.03);48h:(2.04±0.05)vs(0.19±0.01);72h:(0.96±0.02)vs(0.28±0.02);P<0.05],而细胞内IL-6表达水平较同时间点IL-1β组水平显著降低[24h:(0.23±0.01)vs(0.77±0.03);48h:(0.28±0.02)vs(1.35±0.04);72h:(0.20±0.02)vs(1.04±0.04);P<0.05]。心肌素干扰组VSMCs中CGRP处理后各个时间点细胞内IL-6水平较CGRP组显著升高[24h:(1.74±0.04)vs(0.51±0.01);48h:(1.68±0.03)vs(0.29±0.01);72h:(0.98±0.02)vs(0.31±0.01);P<0.05],且CGRP8-37组与CGRP组比较也获得类似结果,而心肌素干扰组和CGRP8-37组中同时间点的细胞内IL-6水平无明显差异。结论CGRP通过促进VSMCs心肌素的表达而抑制IL-1β诱导的细胞炎症激活蛋白IL-6表达,且这一作用是CGRP通过与其受体结合后实现的。

References

[1]  Zheng X L. Myocardin and smooth muscle differentiation [J]. Arch Biochem Biophys, 2014, 543: 48-56. [2]Huang J, Wang T, Wright A C, et al. Myocardin is required for maintenance of vascular and visceral smooth muscle homeostasis during postnatal development[J]. Proc Natl Acad Sci U S A, 2015, 112(14): 4447-4452. [3]陈攀科, 石蓓, 龙仙萍, 等. CGRP修饰大鼠MSCs对血管平滑肌细胞增殖及表型转化的影响[J]. 中国病理生理杂志, 2013, 29(10): 1777-1782. [4]Wang D, Chang P S, Wang Z, et al. Activation of cardiac gene expression by myocardin, a transcriptional cofactor for serum response factor[J]. Cell, 2001, 105(7): 851-862. [5]Minami T, Kuwahara K, Nakagawa Y, et al. Reciprocal expression of MRTF-A and myocardin is crucial for pathological vascular remodelling in mice[J]. EMBO J, 2012, 31(23): 4428-4440. [6]Starke R M, Ali M S, Jabbour P M, et al. Cigarette smoke modulates vascular smooth muscle phenotype: implications for carotid and cerebrovascular disease[J]. PLoS One, 2013, 8(8): e71954. [7]van-der-Veer E P, de-Bruin R G, Kraaijeveld A O, et al. Quaking, an RNA-binding protein, is a critical regulator of vascular smooth muscle cell phenotype[J]. Circ Res, 2013, 113(9): 1065-1075. [8]Talasila A, Yu H, Ackers-Johnson M, et al. Myocardin regulates vascular response to injury through miR-24/-29a and platelet-derived growth factor receptor-beta[J]. Arterioscler Thromb Vasc Biol, 2013, 33(10): 2355- 2365. [9]Lei H, Wu D, Wang J Y, et al. C1q/tumor necrosis factor-related protein-6 attenuates post-infarct cardiac fibrosis by targeting RhoA/MRTF-A pathway and inhibiting myofibroblast differentiation [J]. Basic Res Cardiol, 2015, 110(4): 35. [10]Sisson T H, Ajayi I O, Subbotina N, et al. Inhibition of myocardin-related transcription factor/serum response factor signaling decreases lung fibrosis and promotes mesenchymal cell apoptosis[J]. Am J Pathol, 2015, 185(4): 969-986. [11]Lovren F, Pan Y, Quan A, et al. MicroRNA-145 targeted therapy reduces atherosclerosis[J]. Circulation, 2012, 126(11 Suppl 1): S81-S90. [12]Ackers-Johnson M, Talasila A, Sage A P, et al. Myocardin regulates vascular smooth muscle cell inflammatory activation and disease [J]. Arterioscler Thromb Vasc Biol, 2015, 35(4): 817-828. [13]Diener H C. CGRP as a new target in prevention and treatment of migraine[J]. Lancet Neurol, 2014, 13(11): 1065- 1067. [14]Capuano A, Greco M C, Navarra P, et al. Correlation between algogenic effects of calcitonin-gene-related peptide (CGRP) and activation of trigeminal vascular system, in an ?in vivo? experimental model of nitroglycerin-induced sensitization [J]. Eur J Pharmacol, 2014, 740: 97-102. [15]Mai T H, Wu J, Diedrich A, et al. Calcitonin gene-related peptide (CGRP) in autonomic cardiovascular regulation and vascular structure[J]. J Am Soc Hypertens, 2014, 8(5): 286-296. [16]Kroeger I, Erhardt A, Abt D, et al. The neuropeptide calcitonin gene-related peptide (CGRP) prevents inflammatory liver injury in mice[J]. J Hepatol, 2009, 51(2): 342-353.
[2]  郑林丰,谢应桂,许愿忠,等.大鼠坐骨神经结扎后降钙素基因相关肽的变化[J].第三军医大学学报,2006,28(17):1791. [2]蔡宇红,孙国成,毛云英,等.先天性心脏病肺动脉高压患儿血浆ET-1、CGRP、TXA2及肺组织中其受体的变化[J].第三军医大学学报,2010,32(03):253.  Cai Yuhong,Sun Guocheng,Mao Yunying,et al.Changes of plasma ET-1, CGRP, TXA2 and their receptor expression in lung tissue of children with congenital heart disease-associated pulmonary artery hypertension[J].J Third Mil Med Univ,2010,32(20):253. [3]韩娜,张殿英,王天兵,等.降钙素基因相关肽对大鼠胫骨骨折早期愈合的影响[J].第三军医大学学报,2008,30(21):2011.  HAN Na,ZHANG Dian-ying,WANG Tian-bing,et al.Effects of calcitonin gene-related peptide on tibial fracture healing at early stage in rats[J].J Third Mil Med Univ,2008,30(20):2011. [4]周燕,张纲,卓贤露,等.降钙素基因相关肽对兔成骨细胞增殖和TGF-β1表达的影响[J].第三军医大学学报,2012,34(05):412.  Zhou Yan,Zhang Gang,Zhuo Xianlu,et al.Calcitonin gene-related peptide induces osteoblasts proliferation by un-regulating tumor necrosis factor-β1[J].J Third Mil Med Univ,2012,34(20):412. [5]王少华,许峰,党红星.降钙素基因相关肽对高氧暴露胎鼠原代肺泡Ⅱ型细胞的保护作用及其Wnt信号机制[J].第三军医大学学报,2012,34(09):836.  Wang Shaohua,Xu Feng,Dang Hongxing.Calcitonin gene-related peptide protects premature rat primary type Ⅱ alveolar epithelial cells against damage induced by hyperoxia[J].J Third Mil Med Univ,2012,34(20):836. [6]赵然尊,龙仙萍,刘志江,等.降钙素基因相关肽抑制动脉损伤后NF-κB表达及其对新生内膜增殖的影响[J].第三军医大学学报,2014,36(14):1472.  Zhao Ranzun,Long Xianping,Liu Zhijiang,et al.Effect of calcitonin gene-related peptide on NF-κB expression and neointima formation after artery injury in vivo and in vitro[J].J Third Mil Med Univ,2014,36(20):1472.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133