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CS-US非配对刺激易化豚鼠经典瞬目条件反射的建立

, PP. 1960-1965

Keywords: 瞬目条件反射,条件刺激,非条件刺激,豚鼠

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Abstract:

目的探讨在豚鼠经典瞬目条件反射(eyeblinkconditioning,EBC)中,条件刺激(conditionedstimulus,CS)与非条件刺激(unconditionedstimulus,US)先期的非配对训练对后续的CS-US配对训练结果是否存在易化效应,并分析其可能的神经机制。方法采用96只(4~5月龄,体质量500~550g)健康雄性英国种白化豚鼠,以内侧前额叶或海马的微电流刺激作为中枢CS(mPFCCS或Hip.CS),以纯音刺激作为外周CS(ToneCS),以角膜吹气作为US,对实验组(8组,每组4只动物)和对照组(8组,每组8只动物)进行延迟性瞬目条件反射(delayEBC,DEBC)和痕迹性瞬目条件反射(traceEBC,TEBC)行为训练。其中实验组首先接受1周的非配对训练,包括中枢CS(mPFCCS或Hip.CS)及外周听觉CS(ToneCS)分别与角膜吹气的非配对训练,然后转换CS类型并进行与角膜吹气的配对训练,对照组直接接受中枢或外周CS与角膜吹气配对训练。结果在ToneCS与mPFCCS转换的情况下,无论对于DEBC还是TEBC模式,各实验组条件反应(conditionedresponses,CR)的习得速率均快于对照组(P<0.05)。在ToneCS与Hip.CS转换的情况下,只有在TEBC模式下实验组的CR习得速率快于对照组(P<0.01);而在DEBC模式下,实验组习得CR的速率与对照组无显著差异(P>0.05)。结论先期的CS、US的非配对刺激对于后续EBC建立具有易化效应。两类非特异性刺激可能均不同程度地参与对于EBC的易化作用。

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