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犬肾脏脱细胞基质凝胶的制备及生物相容性实验研究

, PP. 1942-1945

Keywords: 肾脏,脱细胞基质,凝胶,组织工程

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Abstract:

目的制备犬肾脏脱细胞基质凝胶,检测肾脏脱细胞基质凝胶与肾小管上皮细胞的生物相容性。方法取10~12kg成年雄性比格犬肾脏,进行脱细胞处理,制备肾脏脱细胞基质凝胶,利用HE染色DAPI染色观察其是否有细胞残留,扫描电镜观察结构,进行DNA、糖胺聚糖(GAGs)含量检测,对肾小管上皮细胞株(HK-2细胞)进行培养,检测肾脏脱细胞基质凝胶的生物相容性。结果脱细胞处理后的肾脏组织仅留下细胞外基质,无明显细胞核残留;制备的脱细胞基质凝胶呈白色半透明胶状,扫描电镜下呈网状、多孔结构,富含GAGs,并有效去除DNA,体外促进HK-2细胞的生长。结论成功制备了犬肾脏脱细胞基质凝胶,该凝胶能够促进肾小管上皮细胞的生长。

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