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硅橡胶的表面性能与细菌黏附的关系

, PP. 1921-1925

Keywords: 生物材料,感染,细菌黏附,表面性能,亲/疏水性

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Abstract:

目的研究硅橡胶的表面性能变化对细菌黏附的影响。方法采用细菌培养和菌落技术对普通硅橡胶(siliconrubber,SR)、碳-硅橡胶(carbonionimplantedsiliconrubber,C-SR)和羟基磷灰石涂层硅橡胶(hydroxyapatitecoatingsiliconerubber,HA/SR)表面细菌黏附情况进行分析,通过扫描电镜(scanningelectronmicroscope,SEM)对材料表面的形貌进行观察,采用X射线光电子能谱仪(X-rayphotoelectronspectroscopy,XPS)、X射线衍射光谱仪(XRD)、傅里叶红外光谱仪(Fouriertransforminfraredspectrometer,FTIR)对材料表面的分子结构进行分析,同时对材料表面的粗糙度以及亲/疏水性(水接触角)进行检测。结果普通硅橡胶(SR)表面细菌黏附最多[(2.11±0.12)×105CFU],羟基磷灰石涂层硅橡胶(hydroxyapatitecoatingsiliconerubber,HA/SR)次之[(1.88±0.88)×105CFU],碳-硅橡胶(carbonionimplantedsiliconrubber,C-SR)表面细菌黏附最少[(0.98±0.35)×105CFU],C-SR组细菌黏附明显少于SR组(P<0.05),HA/SR组细菌黏附较SR组有所减少,但是差异无统计学意义(P>0.05);材料的表面亲/疏水性与细菌黏附呈相似趋势;而表面粗糙度的变化与细菌黏附趋势不一致。结论硅橡胶表面的亲/疏水性可能是影响细菌黏附的主要因素。

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