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K562细胞对树突状细胞线粒体功能和能量代谢状态的影响

, PP. 1854-1858

Keywords: 树突状细胞,K细胞,线粒体膜电位,线粒体酶活力,傅立叶变换红外光谱

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Abstract:

目的探讨共培养条件下血液肿瘤K562细胞对树突状细胞线粒体功能和能量代谢状态的影响。方法采用免疫磁珠分离方法从健康人外周血中分离纯化出单核细胞,用细胞因子GM-CSF和IL-4将单核细胞诱导分化为未成熟DCs(immatureDCs,imDCs),再利用细胞因子TNF-α进一步将imDCs诱导为成熟DCs(matureDCs,mDCs),在Transwell系统中分别将imDCs和mDCs与K562细胞共培养48h,用流式细胞术检测K562细胞对DCs的线粒体膜电位、细胞内钙离子浓度的影响,采用MTT比色法检测线粒体酶活力变化,用傅立叶变换红外光谱(fouriertransforminfraredspectroscopy,FTIR)技术检测细胞能量代谢状态。对照组为正常培养和撤细胞因子培养48h的DCs。结果与K562细胞共培养后,和对照组DCs相比,imDCs和mDCs的线粒体膜电位下降(P<0.05),imDCs和mDCs胞内钙离子浓度增加(P<0.01),imDCs和mDCs的线粒体酶活力下降(P<0.05,P<0.01),imDCs和mDCs的细胞能量代谢减低(P<0.01),而正常培养组和撤细胞因子后再培养48h的DCs组相比无显著差别。结论与K562细胞共培养后DCs的线粒体功能受到损伤,细胞能量代谢受到抑制,K562细胞可能通过损伤DCs的线粒体功能,抑制细胞能量代谢状态来损伤其迁移能力,进而损伤其免疫功能,从而使血液肿瘤细胞逃脱机体免疫监视,实现血液肿瘤细胞的增殖、扩散和转移。

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