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慢性脑低灌注后失交感神经支配对脑动脉生成的影响

, PP. 1818-1822

Keywords: 慢性脑低灌注,脑动脉生成,交感神经,失交感神经支配

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Abstract:

目的探讨在慢性脑低灌注情况下,失交感神经支配对脑动脉生成的影响。方法通过结扎右侧颈总动脉8周建立慢性脑低灌注模型,外科手术法去除同侧颈上交感神经节。将84只健康的成年雄性SD大鼠用随机数字表法分为动脉结扎+去神经组(联合组)、动脉结扎组、去神经组和假手术组(每组21只)。术后8周,将每组动物随机分成A、B、C3个亚组(每组7只),分别行乳胶灌注、大脑中动脉永久性阻塞以及免疫组化染色。结果联合组右侧软脑膜吻合支直径明显小于动脉结扎组,大于去神经组和假手术组(P<0.05);联合组右侧Willis动脉环直径明显小于动脉结扎组(P<0.05);免疫组化染色结果显示,联合组右侧软脑膜吻合支管壁厚度小于动脉结扎组(P<0.05);8周后行永久性大脑中动脉阻塞实验,联合组显示大脑中动脉区的脑血流值与基线值的比值小于动脉结扎组(P<0.05);神经行为学评分结果显示联合组评分高于动脉结扎组(P<0.05);同时TTC染色显示联合组脑梗死体积百分率大于动脉结扎组(P<0.05)。结论脑血管失交感神经支配损害了慢性脑低灌注时的脑动脉生成。

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