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氟化钠诱导氧化应激促进大鼠子宫组织炎症反应及其作用机制

, PP. 1744-1749

Keywords: 氟化钠,氧化应激,P,JNK信号通路,子宫,炎症反应

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Abstract:

目的通过对氟化钠染毒大鼠的炎症反应相关因子及关键信号通路分子的检测,探讨其对大鼠子宫组织细胞氧化损伤的作用机制。方法将30只雌性SD成年大鼠分为3组(10只/组),分别给予蒸馏水(对照组)、100mg/L氟化钠溶液(100mg/L氟化钠处理组)、200mg/L氟化钠溶液(200mg/L氟化钠处理组)饮用,持续染毒180d,建立氟化钠染毒动物模型。采用Westernblot、免疫组织化学法检测子宫组织炎症相关因子Cox-2、ICAM-1、TNF-α、IL-1β表达;采用ELISA对氧化应激相关分子ROS、MDA、SOD、CAT、GSH进行检测;采用Westernblot测定P38、ERK、JNK、PI3K活性物质的表达。结果2个氟化钠处理组大鼠子宫组织炎症相关因子Cox-2、ICAM-1、TNF-α、IL-1β表达量明显高于对照组(P<0.05);2个氟化钠处理组ROS、SOD、CAT、GSH明显高于对照组,而MDA明显低于对照组(P<0.05);与对照组相比,2个氟化钠处理组P38、JNK蛋白磷酸化效率增加(P<0.05),而且ERK、PI3K总蛋白及磷酸化蛋白表达水平均有增加。结论氟化钠可诱导大鼠子宫组织的氧化应激,促进炎症反应发生,MAPK(ERK、JNK、P38)和PI3K信号通路可能参与其中。

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