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自噬在实验性自身免疫性脑脊髓炎小鼠中的抗炎作用

, PP. 1624-1628

Keywords: 实验性自身免疫性脑脊髓炎,自噬,小胶质细胞,IL-&beta

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Abstract:

目的探索自噬(autophagy)在小鼠实验性自身免疫性脑脊髓炎(experimentalautoimmuneencephalomyelitis,EAE)病程中的时序变化,并初步探讨其在EAE中的抗炎作用及可能机制。方法20只C57BL/6雌性小鼠建模后分别于3、7、16、30d用Westernblot检测中枢神经系统中LC3B的变化。另将60只小鼠分为正常对照组、EAE模型组、EAE+3甲基腺嘌呤(3-Methyladenine,3-MA)组。神经功能评分观察各组小鼠神经功能变化。免疫组化检测各组小鼠免疫后7d小胶质细胞激活情况,Westernblot检测免疫后16、30d白介素-1β(IL-1β)的表达,HE染色观察中枢神经系统炎性细胞浸润情况。结果①EAE+3-MA组小鼠神经功能缺损症状较其他组严重(P<0.05)。②EAE小鼠LC3-Ⅱ/LC3-Ⅰ自免疫后7d开始降低(P<0.05),此后一直呈低表达水平(P<0.05)。③EAE免疫后7d即有小胶质细胞激活,EAE+3-MA组EAE小鼠小胶质细胞激活程度较EAE模型组严重(P<0.05)。④HE染色结果显示,EAE+3-MA组炎细胞浸润比同时间点EAE模型组加重(P<0.05)。⑤免疫后16、30d,EAE模型组高表达IL-1β,EAE+3-MA组小鼠IL-1β表达水平较同时间点EAE模型组高(P<0.05)。结论EAE中存在着自噬水平降低,可能是导致疾病炎症反应发生的重要原因。

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