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CD25siRNA基因转染可提高大鼠穿透性角膜移植片的存活率

, PP. 1543-1549

Keywords: CDsiRNA,移植排斥反应,纳米转染试剂,IL-,FOXP

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Abstract:

目的探讨CD25siRNA基因转染对大鼠穿透性角膜移植排斥反应的作用,及术后植片中IL-10和FOXP3的含量变化。方法配制50μLEntransterTM-反义CD25siRNA和50μLEntransterTM-CD25siRNA点眼纳米转染试剂,50μL生理盐水为对照。建立Wistar-SD大鼠穿透性角膜移植模型,右眼为术眼,采用单纯随机抽样方法,分为对照组(A组)、EntransterTM-反义CD25siRNA转染组(B组)和EntransterTM-CD25siRNA转染组(C组),每组28只。术后每日对植片行临床评分并分别于3、7、14、21d行HE染色、免疫组化、Westernblot和qRT-PCR检测。结果角膜植片生存曲线显示CD25siRNA转染组角膜植片的生存率显著优于其余2组(P<0.05);HE染色显示CD25siRNA转染组较其余2组,角膜植片新生血管减少,炎症细胞渗入减少;同时,术后各时间点CD25siRNA转染组中CD25表达均低于其余2组(P<0.05),但IL-10表达均高于其余2组(P<0.05),FOXP3表达在3组间无统计学意义(P>0.05)。结论CD25siRNA转染通过上调抗炎性细胞因子IL-10表达,抑制炎症反应,进而延长角膜植片生存时间,提高植片生存率。

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