World Health Organization. WHO manual for the standardized investigation and diagnosis of the infertile couple[M]. Cambridge:? Cambridge University Press, 2000: 379-383. [2]Pasqualotto F F, Pasqualotto E B, Sobreiro B P, et al. Clinical diagnosis in men undergoing infertility investigation in a university hospital[J]. Urol Int, 2006, 76(2): 122-125. [3]Tantibhedhyangkul J, Hawkins K C, Dai Q, et al. Expression of a mitochondrial progesterone receptor in human spermatozoa correlates with a progestin-dependent increase in mitochondrial membrane potential[J]. Andrology, 2014, 2(6): 875-883. [4]史孟婧, 王健, 但国蓉, 等. 缺氧对PC12细胞中miR-210的表达及对细胞线粒体功能的影响[J]. 第三军医大学学报, 2014, 36(10): 979-982. [5]Shin S M, Kim S, Hong J G, et al. IGF-I improves mitochondrial membrane potential during hypothermic storage of canine spermatozoa[J]. J Vet Med Sci, 2014, 76(7): 1065-1067. [6]Jiang S, Yu L, Cheng J, et al. Genomic damages in peripheral blood lymphocytes and association with polymorphisms of three glutathione S-transferases in workers exposed to formaldehyde[J]. Mutat Res, 2010, 695(1/2): 9-15. [7]World Health Organization. WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interactions[M]. 4th ed. Cambridge:? Cambridge University Press, 1999. [8]Tang K, Xue W, Xing Y, et al. Genetic polymorphisms of glutathione S-transferase M1, T1, and P1, and the assessment of oxidative damage in infertile men with varicoceles from northwestern China[J]. J Androl, 2012, 33(2): 257-263. [9]Sharbatoghli M, Valojerdi M R, Amanlou M, et al. Relationship of sperm DNA fragmentation, apoptosis and dysfunction of mitochondrial membrane potential with semen parameters and ART outcome after intracytoplasmic sperm injection[J]. Arch Gynecol Obstet, 2012, 286(5): 1315-1322. [10]Ghaleno L R, Valojerdi M R, Janzamin E, et al. Evaluation of conventional semen parameters, intracellular reactive oxygen species, DNA fragmentation and dysfunction of mitochondrial membrane potential after semen preparation techniques:? a flow cytometric study[J]. Arch Gynecol Obstet, 2014, 289(1): 173-180. [11]谭迎春, 陈子江, 卢少明, 等. 用荧光探针JC-1检测氧化应激对精子线粒体膜电位的影响[J].山东大学学报: 医学版, 2006, 44(5): 447-450. [12]Hayes J D, Flanagan J U, Jowsey I R. Glutathione transferases[J]. Annu Rev Pharmacol Toxicol, 2005, 45: 51-88. [13]Ramio-Lluch L, Yeste M, Fernandez-Novell J M, et al. Oligomycin A-induced inhibition of mitochondrial ATP-synthase activity suppresses boar sperm motility and ?in vitro? capacitation achievement without modifying overall sperm energy levels[J]. Reprod Fertil Dev, 2014, 26(6): 883-897. ?