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一种昆明种属来源的视网膜变性小鼠同源导入C57BL/6J小鼠近交系的表型研究

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Keywords: 视网膜色素变性,视网膜电图,同源导入近交系,表型,发育?

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Abstract:

目的将本实验室发现的一种昆明种属来源的自发性遗传性视网膜变性小鼠(暂命名为KMrdf/rdf小鼠)的致病基因同源导入C57BL/6J小鼠,培育一种新的近交系小鼠(暂命名为B6rdf/rdf小鼠),并对B6rdf/rdf小鼠进行眼部表型的发育学研究。方法采用眼底照相、眼底荧光素血管造影、组织形态学和视网膜电图观测不同年龄B6rdf/rdf小鼠的眼部表型。结果眼底照相和荧光素血管造影发现B6rdf/rdf小鼠表现为进行性视网膜血管退化及脱色素改变,视网膜组织形态学表现为外核层在P14显著变薄,外核层和外网状层至P21已基本消失,视网膜电图自P14已记录不到明显波形。结论B6rdf/rdff小鼠眼部表型呈快速进行性改变,与KMrdf/rdf小鼠表型相似。

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