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过表达MORC2通过下调p53含量抑制L02肝细胞脂肪变性

, PP. 962-967

Keywords: 非酒精性脂肪性肝病,脂肪变性,L肝细胞,MORC,p

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Abstract:

目的研究过表达MORC2对人L02肝细胞脂肪变性程度的影响及可能机制。方法利用慢病毒转染技术在L02肝细胞中过表达MORC2,qRT-PCR及Westernblot鉴定过表达效果后,对L02肝细胞进行脂肪变性诱导,测定细胞甘油三酯(triglyceride,TG)含量并比较过表达前后L02肝细胞的脂肪蓄积程度;利用芯片技术对过表达MORC2前后的基因表达谱进行检测与Pathway分析,筛选过表达MORC2时发生变化的通路并选择对脂代谢有调控作用的p53进行研究;Westernblot检测在L02肝细胞脂肪变性诱导时过表达MORC2对p53蛋白含量的影响;在过表达MORC2的基础上对L02肝细胞过表达p53,qRT-PCR及Westernblot鉴定p53过表达效果后,通过TG测定观察过表达p53对过表达MORC2改变的L02肝细胞脂肪蓄积程度产生的影响。结果qRT-PCR及Westernblot证明过表达MORC2成功;过表达MORC2的L02肝细胞在脂肪变性诱导后TG含量较MORC2正常表达的L02肝细胞明显降低(P<0.01);基因表达谱芯片及Pathway分析显示脂代谢调控相关的p53通路在过表达MORC2后发生变化;Westernblot显示过表达MORC2后,L02肝细胞中p53蛋白水平在脂肪变性诱导前后均降低;qRT-PCR及Westernblot验证p53过表达成功,且过表达p53可部分解除MORC2对L02肝细胞脂肪变性的缓解作用。结论过表达MORC2可通过下调p53从而缓解肝细胞脂肪变性。

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