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过表达精子相关抗原9对前列腺癌细胞PC3生物学行为的影响

, PP. 1012-1016

Keywords: SPAG,前列腺癌,增殖,细胞周期

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Abstract:

目的研究精子相关抗原9(sperm-associatedantigen9,SPAG9)对前列腺癌细胞增殖、细胞周期、侵袭转移等生物学行为的影响,探讨可能的分子机制。方法前列腺细胞PC3中转染SPAG9后,qRT-PCR和Westernblot检测转染后细胞SPAG9的表达变化;CCK-8检测SPAG9对PC3细胞增殖的影响;流式细胞术检测SPAG9对PC3细胞周期的作用;Transwell实验检测转染SPAG9对PC3细胞体外迁移能力的影响。结果qRT-PCR检测结果表明SPAG9组的SPAG9的相对表达量(20.7763±3.0809)明显高于对照组(1.0102±0.1784)(P<0.01)。Westernblot结果显示在前列腺癌细胞PC3中过表达SPAG9后成功。CCK-8实验表明SPAG9促进细胞增殖;流式细胞术检测结果表明过表达SPAG9使PC3细胞周期加快;在Transwell迁移实验中,SPAG9转染细胞后,SPAG9组穿膜细胞数为(88.078±4.509)/视野,对照组穿膜细胞数为(54.333±4.582)/视野,SPAG9组细胞迁移能力明显高于对照组(P<0.05)。结论SPAG9促进前列腺癌细胞PC3的增殖、细胞周期及侵袭转移等生物学行为,并可能通过ERK和JNK信号通路发挥这些作用。

References

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