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地塞米松增强L02细胞P-糖蛋白的表达作用机制的初步研究

, PP. 858-862

Keywords: L细胞,PIK/Akt,P-糖蛋白,地塞米松

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Abstract:

目的探讨地塞米松对人正常肝细胞株L02细胞P-糖蛋白(P-gp)表达的影响,以及磷酸肌醇-3激酶(PI3K)/蛋白激酶B(Akt)途径在调控P-gp表达中的作用。方法采用Westernblot检测体外培养L02细胞P-gp与Akt的表达水平,比较经地塞米松、PI3K/Akt酶抑制剂LY294002及两者联合处理24h前后L02细胞P-gp表达水平的变化及地塞米松处理24h前后L02细胞Akt表达水平的差异,分析L02细胞P-gp表达与PI3K/Akt表达之间的关系。结果10μmol/L地塞米松刺激24h后L02细胞P-gp及Akt表达水平较刺激前分别显著增加60.8%(P=0.0267)和25.0%(P=0.0358);L02细胞中P-gp表达水平与Akt表达水平之间呈显著正相关(Pearson系数=0.87,P<0.01);LY294002(20μmol/L)处理组L02细胞P-gp表达水平较对照组(未处理组)显著降低33.8%(P=0.0184);地塞米松单独处理组和地塞米松与LY294002联合处理组L02细胞P-gp表达水平分别是对照组的(163.5±18.4)%和(130.1±22.0)%,两者间相差显著(P=0.0313)。结论地塞米松可能通过激活PI3K/Akt途径诱导L02细胞P-gp增强表达。

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