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川西獐牙菜单体齐墩果酸上调阻塞性胆汁淤积大鼠肝脏核受体Lrh-1和胆酸转运蛋白Mrp3的表达

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Keywords: 胆汁淤积,齐墩果酸,多药耐药相关蛋白,转录因子

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Abstract:

目的建立胆道结扎的大鼠模型,观察川西獐牙菜活性单体齐墩果酸(oleanolicacid,OA)对胆道结扎所致的肝外胆汁淤积的保护作用,及其对多药耐药相关蛋白3(multidrugresistance-associatedprotein3,Mrp3)、核受体Lrh-1的调控作用。方法采用随机数字表法将15只SD大鼠分为3组:假结扎组(Sham组)、胆道结扎组(BDL组)、齐墩果酸组(BDL+OA组),每组5只。分别用生理盐水、齐墩果酸处理7d后,收集组织标本,HE染色检测肝脏损伤程度,Westernblot及RT-PCR检测Mrp3和Lrh-1在蛋白及mRNA水平的变化。结果HE染色结果显示BDL+OA组肝脏损伤程度较胆道结扎组明显减轻;Westernblot结果表明BDL+OA组Mrp3表达较Sham组增加2.4倍,Lrh-1表达增加1.8倍;RT-PCR结果显示BDL+OA组Mrp3表达较Sham组增加3.2倍,Lrh-1表达升高2.2倍。结论川西獐牙菜单体齐墩果酸对胆道结扎所致大鼠胆汁淤积性肝损伤具有保护作用,而这种保护作用可能与Lrh-1、Mrp3有关。

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