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Lysine-CyclicRGD肽对骨髓间充质干细胞黏附、增殖和分化的影响

, PP. 713-717

Keywords: 组织工程,支架材料,细胞黏附,生物材料,表面修饰

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Abstract:

目的研究物理电荷与生物配基两种因素相结合的新型表面修饰剂Lysine-CyclicRGD(LcRGD)肽对骨髓间充质干细胞(mesenchymalstemcells,MSCs)的黏附、增殖及分化的影响。方法以光学接触角仪衡量LcRGD肽修饰表面的亲水性;以离心黏附实验、震荡黏附实验评价LcRGD肽对MSCs的黏附性能;以CCK-8实验评价LcRGD表面改性DBM上MSCs的增殖活力;以Westernblot法检测LcRGD表面改性脱钙骨基质(decalcifiedbonematrix,DBM)上MSCs的Runx2和OCN蛋白的表达。结果LcRGD肽修饰表面接触角为(29.33±4.06)°,远低于普通cRGD肽修饰表面的接触角(63.72±8.65)°,提示LcRGD肽具有更好的亲水性(P<0.01);离心黏附实验和震荡黏附实验显示相对于普通cRGD肽,LcRGD肽从细胞黏附早期开始便具有更好的细胞黏附性能;CCK-8实验提示MSCs在LcRGD修饰的DBM支架材料上的增殖活力高于普通cRGD肽修饰的DBM支架材料(P<0.05);Westernblot结果提示相对于普通cRGD肽修饰的DBM,MSCs在LcRGD修饰的DBM上表达更高的成骨标志蛋白水平,提示LcRGD肽具有更好的骨诱导性。结论相对于普通的cRGD肽,物理电荷与生物配基相结合的LcRGD肽具有更好的细胞黏附、促增殖和骨诱导性能。

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