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宫颈鳞癌组织中HPVE6与野生型p53和PinX1表达的关系及其临床意义

, PP. 782-786

Keywords: 宫颈鳞癌,HPVE,野生型p,PinX

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Abstract:

目的探讨宫颈鳞癌、宫颈鳞癌癌旁及正常宫颈组织中HPVE6、野生型p53及PinX1表达及其关系。方法收集第三军医大学大坪医院野战外科研究所妇产科2013年3月至2014年2月17例宫颈鳞癌患者宫颈鳞癌、宫颈鳞癌癌旁组织,选取10例正常宫颈组织作为对照。采用Real-TimePCR、Westernblot、免疫组织化学方法检测3组组织中HPVE6、野生型p53和PinX1的mRNA、蛋白的表达及分布。结果宫颈鳞癌组织中mRNA及蛋白水平结果均显示HPVE6的表达水平显著高于癌旁组织(P<0.01),而野生型p53和PinX1的表达水平明显低于癌旁及正常组织(P<0.05)。相关性分析提示:宫颈鳞癌组织中野生型p53(R=-0.605,P<0.01)和PinX1(R=-0.496,P<0.05)表达水平的下降与HPVE6的表达水平升高存在负相关,野生型p53与PinX1的表达呈正相关(R=0.824,P<0.01)。结论宫颈鳞癌组织中HPVE6表达上调与野生型p53、PinX1的表达负相关,PinX1的表达与野生型p53密切相关,HPVE6可能通过抑制野生型p53、PinX1导致宫颈鳞癌的发生、发展。

References

[1]  Woodman C B, Collins S I, Young L S. The natural history of cervical HPV infection: unresolved issues [J]. Nat Rev Cancer, 2007, 7(1): 11-22. [2]Ghittoni R, Accardi R, Hasan U, et al. The biological properties of E6 and E7 oncoproteins from human papillomaviruses [J]. Virus Genes, 2010, 40(1): 1-13. [3]Jiang P, Yue Y. Human papillomavirus oncoproteins and apoptosis (Review) [J]. Exp Ther Med, 2014, 7(1): 3-7. [4]Jiang J, Zhao L J, Zhao C, et al. Hypomethylated CpG around the transcription start site enables TERT expression and HPV16 E6 regulates TERT methylation in cervical cancer cells[J]. Gynecol Oncol, 2012, 124(3): 534-541. [5]Yoo J E, Park Y N, Oh B K. PinX1, a telomere repeat-binding factor 1 (TRF1)-interacting protein, maintains telomere integrity by modulating TRF1 homeostasis, the process in which human telomerase reverse Transcriptase (hTERT) plays dual roles [J]. J Biol Chem , 2014, 289(10): 6886-6898. [6]Lai X F, Shen C X, Wen Z, et al. PinX1 regulation of telomerase activity and apoptosis in nasopharyngeal carcinoma cells [J]. J Exp Clin Cancer Res, 2012, 31: 12. [7]Wu G, Liu D, Jiang K, et al. PinX1, a novel target gene of p53, is suppressed by HPV16 E6 in cervical cancer cells [J]. Biochim Biophys Acta , 2014, 1839(2): 88-96. [8]Kjaer S K, Munk C, Junge J, et al. Carcinogenic HPV prevalence and age-specific type distribution in 40, 382 women with normal cervical cytology, ASCUS/LSIL, HSIL, or cervical cancer: what is the potential for prevention?[J]. Cancer Causes Control, 2014, 25(2): 179-189. [9]Meijer C J, Snijders P J. Cervical cancer in 2013: Screening comes of age and treatment progress continues[J]. Nat Rev Clin Oncol, 2014, 11(2): 77-78. [10]Smeenk L, van-Heeringen S J, Koeppel M, et al. Role of p53 serine 46 in p53 target gene regulation [J]. PLoS One, 2011, 6(3): e17574. [11]Denk C, Butz K, Schneider A, et al. p53 mutations are rare events in recurrent cervical cancer [J]. J Mol Med (Berl), 2001, 79(5/6): 283-288. [12]Massimi P, Shai A, Lambert P, et al. HPV E6 degradation of p53 and PDZ containing substrates in an E6AP null background [J]. Oncogene, 2008, 27(12): 1800-1804. [13]Li M, He Y, Feng X, et al. Genome-wide studies of the transcriptional regulation by p53 [J]. Biochim Biophys Acta, 2012, 1819(7): 684-687. [14]Shi M, Du L, Liu D, et al. Glucocorticoid regulation of a novel HPV-E6-p53-miR-145 pathway modulates invasion and therapy resistance of cervical cancer cells [J]. J Pathol, 2012, 228(2): 148-157. [15]Soohoo C Y, Shi R, Lee T H, et al. Telomerase inhibitor PinX1 provides a link between TRF1 and telomerase to prevent telomere elongation [J]. J Biol Chem, 2011, 286(5): 3894-3906. [16]Yonekawa T, Yang S, Counter C M. PinX1 localizes to telomeres and stabilizes TRF1 at mitosis [J]. Mol Cell Biol, 2012, 32(8): 1387-1395. [17]Zhou X Z, Huang P, Shi R, et al. The telomerase inhibitor PinX1 is a major haploinsufficient tumor suppressor essential for chromosome stability in mice [J]. J Clin Invest, 2011, 121(4): 1266-1282.

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