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OPN诱导小鼠MSCs向表皮细胞分化促进创面愈合的研究

, PP. 594-599

Keywords: 愈合,间充质干细胞(MSCs),骨桥蛋白(OPN),表皮,分化

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Abstract:

目的以骨桥蛋白(osteopontin,OPN)基因敲除小鼠及野生型小鼠为研究对象,探讨骨髓间充质干细胞(mesenchymalstemcells,MSCs)分化为表皮细胞的潜能及骨桥蛋白在分化过程中的作用。方法体外实验:将分离培养的野生型(WT)和基因敲除型(KO)新生鼠(鼠龄1d)MSCs鉴定纯度后分别设为WT组和KO组,取其第3代分别在表皮转化培养体系中孵育,通过镜下观察、流式细胞术、免疫荧光染色、Westernblot等方法,比较两组表皮细胞标志物(CK14)表达水平的差异。动物实验:实验分4组,WT组小鼠创周注射GFP-MSCs(WT/MSCs)组,WT小鼠创周注射PBS(WT/PBS)组,KO小鼠创周注射GFP-MSCs(KO/MSCs)组,KO小鼠创周注射PBS(KO/PBS)组,每组各8只雄鼠,鼠龄6~8周,体质量20~25g,按分组将分离培养的GFP-MSCs和PBS注射到创周,观察创面愈合及GFP-MSCs向表皮细胞分化的情况。结果分离培养的MSCs呈梭形或成纤维细胞样生长,流式细胞术检测MSCs的纯度超过91%;诱导分化第12、17天的两组细胞检测发现,WT组和KO组的细胞均表达CK14;第17天免疫荧光结果示WT组CK14的表达阳性率(0.9363±0.0095)明显高于KO组(0.6475±0.0231),(P<0.01);Westernblot检测结果也显示KO组的CK14表达量(0.3894±0.0168)明显低于WT组(0.6146±0.0153),(P<0.01)。动物实验WT/PBS组创面愈合速度快于KO/PBS组(P<0.05),WT/MSCs组的创面愈合速度快于WT/PBS组(P<0.05),注射的GFP-MSCs向表皮细胞分化的能力WT/MSCs组强于KO/MSCs组(P<0.05)。结论OPN在体内、体外均可诱导MSCs分化为表皮细胞,进而促进创面愈合。

References

[1]  Li M, Yu J, Li Y, et al. CXCR4+ progenitors derived from bone mesenchymal stem cells differentiate into endothelial cells capable of vascular repair after arterial injury[J]. Cell Reprogram, 2010, 12(4): 405-415. [2]Hisada K, Hata K, Ichida F, et al. Retinoic acid regulates commitment of undifferentiated mesenchymal stem cells into osteoblasts and adipocytes[J]. J Bone Miner Metab, 2013, 31(1): 53-63. [3]Barba M, Cicione C, Bernardini C, et al. Adipose-derived mesenchymal cells for bone regereneration: state of the art[J]. Biomed Res Int, 2013, 2013: 416391. [4]Huang K, Kang X, Wang X, et al. Conversion of bone marrow mesenchymal stem cells into type Ⅱ alveolar epithelial cells reduces pulmonary fibrosis by decreasing oxidative stress in rats[J]. Mol Med Rep, 2015, 11(3): 1685- 1692. [5]Knoll A, Brockmeyer T, Chevalier R, et al. Adult rat bone marrow-derived stem cells promote late fetal type Ⅱ cell differentiation in a co-culture model[J]. Open Respir Med J, 2013, 7: 46-53. [6]Rohaina C M, Then K Y, Ng A M, et al. Reconstruction of limbal stem cell deficient corneal surface with induced human bone marrow mesenchymal stem cells on amniotic membrane[J]. Transl Res, 2014, 163(3): 200-210. [7]Jiang T S, Cai L, Ji W Y, et al. Reconstruction of the corneal epithelium with induced marrow mesenchymal stem cells in rats[J]. Mol Vis, 2010, 16: 1304-1316. [8]Li W, Ye B, Cai X Y, et al. Differentiation of human umbilical cord mesenchymal stem cells into prostate-like epithelial cells in vivo[J]. PLoS One, 2014, 9(7): e102657. [9]Meng H, Wang Z, Wang W, et al. Effect of osteopontin in regulating bone marrow mesenchymal stem cell treatment of skin wounds in diabetic mice[J]. Diabetes Metab Res Rev, 2014, 30(6): 457-466. [10]Pacini S, Petrini I. Are MSCs angiogenic cells? New insights on human nestin-positive bone marrow-derived multipotent cells[J]. Front Cell Dev Biol, 2014, 2: 20. [11]Liu X, Wang J A, Ji X Y, et al. Preconditioning of bone marrow mesenchymal stem cells by prolyl hydroxylase inhibition enhances cell survival and angiogenesis ?in vitro? and after transplantation into the ischemic heart of rats[J]. Stem Cell Res Ther, 2014, 5(5): 111. [12]Gomathysankar S, Halim A S, Yaacob N S. Proliferation of keratinocytes induced by adipose-derived stem cells on a chitosan scaffold and its role in wound healing, a review[J]. Arch Plast Surg, 2014, 41(5): 452-457. [13]Hassan W U, Greiser U, Wang W. Role of adipose-derived stem cells in wound healing[J]. Wound Repair Regen, 2014, 22(3): 313-325. [14]Kim W S, Park B S, Sung J H, et al. Wound healing effect of adipose-derived stem cells: a critical role of secretory factors on human dermal fibroblasts[J]. J Dermatol Sci, 2007, 48(1): 15-24. [15]Badiavas E V, Falanga V. Treatment of chronic wounds with bone marrow-derived cells[J]. Arch Dermatol, 2003, 139(4): 510-516. [16]Dabiri G, Heiner D, Falanga V. The emerging use of bone marrow-derived mesenchymal stem cells in the treatment of human chronic wounds[J]. Expert Opin Emerg Drugs, 2013, 18(4): 405-419.

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