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CX43蛋白磷酸化在大鼠逼尿肌过度活动症中的作用

, PP. 495-499

Keywords: CX蛋白,磷酸化,逼尿肌过度活动症

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Abstract:

目的评价CX43蛋白磷酸化在大鼠逼尿肌过度活动症(detrusoroveractivity,DO)中的作用,并初步探讨PP1和PP2A对CX43蛋白磷酸化修饰状态的影响。方法建立大鼠DO模型作为实验组,假手术组作为对照组,Westernblot检测CX43蛋白磷酸化水平、PP1及PP2A的表达变化,免疫荧光双染对CX43与PP1、PP2A的定位及表达变化进行检测,荧光漂白恢复实验(FRAP)比较正常组、DO组、DO+碱性磷酸酶(AP)组的细胞间通讯功能(gapjunctionintercellularcommunication,GJIC)的差异。结果与正常组相比,DO组膀胱中的CX43蛋白的不同磷酸化修饰状态(P0、P1、P2)的表达水平均明显升高(P<0.05),PP2A的表达明显降低(P<0.05),PP1的表达未发现明显改变(P>0.05)。DO组的GJIC较正常组明显增加(P<0.05),DO+AP组的GJIC较正常组和DO组明显降低(P<0.05)。结论CX43蛋白的磷酸化水平上调可能在DO的发病过程中发挥着重要的作用,这种磷酸化水平的上调可能与PP2A的表达减少相关。

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