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MRPS5对膀胱癌细胞增殖能力及干性特征的影响

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Keywords: 膀胱癌,线粒体核糖体蛋白S(MRPS),增殖,肿瘤干细胞

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Abstract:

目的探讨线粒体核糖体蛋白S5(mitochondrialribosomalproteinS5,MRPS5)对人膀胱癌细胞的增殖能力及其中干细胞干性特征的影响。方法Westernblot检测人膀胱癌及癌旁正常组织MRPS5的表达;构建靶向干扰MRPS5的慢病毒载体,感染膀胱癌细胞株T24,以干扰Scramble序列作为对照,Westernblot检测MRPS5干扰效率;细胞活力实验检测细胞增殖能力;流式细胞术检测细胞周期;细胞成球实验观察成球能力;Westernblot检测肿瘤干性转录因子Nanog,Oct4,c-Myc和Sox2的表达;小鼠皮下移植瘤实验观察T24体内成瘤能力。结果MRPS5在膀胱癌组织中的表达高于癌旁正常组织;慢病毒干扰载体PLKO.1-shMRPS5有效,且干扰MRPS5表达后T24细胞增殖能力下降(P<0.05),周期阻滞于S期,干性因子表达均下调,成球率无变化(P>0.05)但成球直径明显减小;同时小鼠体内成瘤体积减小[(0.784±0.278)vs(0.500±0.245)cm3,P<0.05],瘤质量减轻[(0.862±0.372)vs(0.412±0.248)g,P<0.05]。结论MRPS5在膀胱癌中较高表达,且干扰MRPS5表达能抑制T24增殖并抑制T24中的干细胞生物学特征。

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