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二甲基亚砜通过降低炎症和氧化应激改善鱼精蛋白诱导的大鼠膀胱炎

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Keywords: 二甲基亚砜,间质性膀胱炎,鱼精蛋白,膀胱炎

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Abstract:

目的探讨二甲基亚砜(dimethylsulfoxide,DMSO)对鱼精蛋白(protaminesulfate,PS)诱导的大鼠间质性膀胱炎(interstitialcystitis,IC)治疗作用。方法SD雌性大鼠48只,按随机数字表法分为正常组、PS灌注组、PS+DMSO组、盐水灌注组(NS组),每组12只大鼠。正常组大鼠未给予任何处理;PS组用0.5mL,30mg/mL的PS膀胱内灌注并在膀胱内保留30min;PS+DMSO组使用50%DMSO溶解PS,按照0.5mL,30mg/mL的PS膀胱内灌注并在膀胱内保留30min;NS组用0.5mL生理盐水膀胱灌注并保留30min,各组大鼠每周处理1次,连续处理4周。4周后,HE和甲苯胺蓝染色用于病理学评分和肥大细胞计数;尿动力学和肌条实验被用于评价大鼠膀胱功能和肌肉收缩性改变;Westernblot检测SOD2、GSH-Px、IL-6、IL-1B、TNF-α的表达量。结果与PS组相比,PS+DMSO组大鼠膀胱病理学评分[(3.00±0.00)vs(0.83±0.40),P<0.05]、肥大细胞计数[(19.66±2.16)vs(6.16±1.16),P<0.05]以及炎症因子IL-6、IL-1B和TNF-α表达(P<0.05)显著降低,而抗氧化应激酶(SOD2和GSH)显著恢复(P<0.05);在膀胱功能学上,与PS组相比,PS+DMSO组大鼠膀胱排尿频率[(24.83±3.06)vs(9.83±1.47),P<0.05]显著降低,排尿间隔[(2.42±0.37)vs(6.40±0.57),P<0.05]显著延长,肌肉收缩频率[(6.93±0.97)vs(4.94±0.73),P<0.05]显著降低。结论DMSO可能通过降低炎症和氧化应激改善鱼精蛋白诱导的大鼠膀胱炎。

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