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S-雌马酚改善高糖培养条件下INS-1细胞胰岛素分泌功能的研究

, PP. 386-391

Keywords: S-雌马酚,INS-细胞,葡萄糖刺激胰岛素分泌,葡萄糖转运蛋白,解偶联蛋白

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Abstract:

目的研究大豆甙元活性代谢产物S-雌马酚(S-equol,S-Eq)对高糖培养条件下大鼠胰岛素瘤(INS-1)细胞胰岛素分泌功能的影响。方法采用26.2mmol/L葡萄糖(高糖组,H)及葡萄糖分别与不同浓度S-Eq(0.1、1、10、100?ol/LS-Eq+H组)干预INS-1细胞24h,另设未干预的INS-1细胞为对照组(C)。采用CCK-8检测细胞活力,ELISA法测定葡萄糖刺激胰岛素分泌(GSIS)功能,TUNEL法联合AnnexinV-FITC/PI流式细胞术检测细胞凋亡,Real-timePCR和Westernblot分别检测前胰岛素原(preproinsulin,PPI)、葡萄糖转运蛋白2(glucosetransporter2,Glut2)、线粒体阴离子载体解偶联蛋白2(uncouplingprotein2,UCP2)mRNA及蛋白表达。结果S-Eq能显著增加高糖培养条件下INS-1细胞活力(P<0.05),其中1?ol/LS-Eq效果最为明显。GSIS检测发现,与对照组比较,高糖处理后INS-1细胞胰岛素分泌显著降低,而S-Eq能显著增加高糖处理的INS-1细胞的胰岛素分泌(P<0.05)。同时,0.1、1、10?ol/LS-Eq均能明显减少高糖培养条件下INS-1细胞凋亡,上调PPImRNA、Glut2mRNA和Glut2蛋白表达,而显著抑制UCP2mRNA及其蛋白的表达(P<0.05)。结论S-Eq可有效改善高糖培养条件下INS-1细胞胰岛素分泌功能,可能与抑制细胞凋亡,调节Glut2和UCP2表达有关。

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