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T细胞因子3在非小细胞肺癌中的表达

, PP. 425-428

Keywords: T细胞因子/TCF-,非小细胞肺癌,Wnt通路

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Abstract:

目的研究T细胞因子3(Tcellfactor3,TCF-3)在非小细胞肺癌中的表达及分布情况。方法采用50对人非小细胞肺癌组织标本及其正常癌旁组织对照,进行免疫组化检测TCF-3蛋白在肺癌组织及正常肺组织中的表达及分布情况;采用5种人非小细胞肺癌细胞系及2种正常人支气管上皮细胞系进行实时荧光定量PCR,Westernblot检测肺癌细胞系中TCF-3在mRNA和蛋白水平的表达情况。结果(1)TCF-3在肺癌组织中表达,表达率为48%(24/50),在正常肺组织中未见表达。TCF-3在肺癌细胞胞核与胞质同表达,以细胞核表达为主。(2)TCF-3在A549、H520、HBE、BEP-2D4种细胞系中mRNA及蛋白水平均有表达。结论TCF-3在肺癌组织及细胞系中均表达,TCF-3蛋白在细胞核与细胞质同表达,但以细胞核表达为主。

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