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MG53在下肢远程缺血预适应保护肺缺血再灌注损伤中的作用

, PP. 409-413

Keywords: 远程缺血预适应,肺缺血再灌注损伤,MG,大鼠,小鼠

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Abstract:

目的研究MG53在下肢远程缺血预适应保护肺组织缺血再灌注损伤中的作用。方法肺缺血再灌注损伤模型通过开胸阻断左侧肺门进行缺血1h后再灌注1h建立。下肢远程缺血预适应模型是在进行肺缺血再灌注损伤前0.5h阻断左侧股动脉血流5min,然后再灌注5min,重复3个循环来实现的。将60只10~12周龄SD大鼠[雌雄各半,体质量(235±15)g]采用完全随机分组方法分为3组(n=20):假手术组(sham组)、肺缺血再灌注损伤组(IR组)、下肢远程缺血预适应+肺缺血再灌注损伤组(RIP组)。将24只8~12周龄MG53敲除小鼠[雌雄各半,体质量(27.5±2.5)g]采用完全随机分组方法分为2组(n=12),即肺缺血再灌注损伤组(IR组)、下肢远程缺血预适应+肺缺血再灌注损伤组(RIP组)。在缺血及再灌注时间结束后,观察动脉氧分压、计算肺组织湿干比(W/D),通过Westernblot检测3组之间肺组织上MG53蛋白表达量的改变,并记录其生存情况。结果对SD大鼠,在进行肺缺血再灌注损伤后其血氧分压降低到(46.60±5.94)mmHg,而下肢远程缺血预适应能显著改善肺组织的缺血再灌注损伤,使其血氧分压恢复至(73.40±5.94)mmHg(P<0.05),同时对反映肺组织水肿程度的W/D值、生存情况都有显著的改善,通过Westernblot方法检测到RIP组肺组织上MG53蛋白的表达量(2.03±0.53)较IR组(0.79±0.17)显著增加(P<0.05)。在MG53敲除小鼠上,下肢远程缺血预适应组的血氧分压(48.20±12.15)mmHg较肺缺血再灌注损伤组(45.60±10.53)mmHg并未得到改善,同时也不能改善肺组织的水肿情况。结论MG53参与了下肢远程缺血预适应对肺组织缺血再灌注损伤的保护作用。

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