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男性避孕纳米粒的制备与体外实验研究

, PP. 351-355

Keywords: FSHR,PLGA,纳米疫苗,避孕

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Abstract:

目的构建聚乳酸-羟乙酸共聚物(polylacticacid-glycolicacid,PLGA)包载促卵泡生成素受体表位肽(FSHR32-44)的纳米粒,探讨PLGA材料作为避孕疫苗载体的可行性。方法使用PLGA为载体,制备出包载促卵泡生成素受体的B细胞表位(FSHR32-44)的纳米粒,透射电镜(TEM)观察表观形态,粒径仪检测粒径分布范围和Zeta电位,体外释放试验检测其理化特性。激光共聚焦显微镜观察小鼠骨髓来源树突状细胞(DCs)吞噬FSHR可溶性多肽和FSHR/PLGANP的能力,流式细胞仪检测FSHR、PLGANP和FSHR/PLGANP促骨髓来源DCs成熟能力。结果单乳-溶剂挥发法制备的FSHR/PLGANP大小粒径分布均匀、表面规整,粒径(296.3±3.5)nm,Zeta电位(-16.1±0.4)mV,FSHR包封率(62.71±2.83)%,并具有缓慢释放抗原的能力;体外细胞水平实验结果显示FSHR表位肽纳米粒易于被DCs摄取,有剂量和时间依赖性,能显著上调DCs表面CD40、CD86、CD83和MHC-Ⅱ功能分子的表达。结论PLGA包载FSHR多肽有良好的包封率,易被DCs吞噬,并有强的促进DCs成熟作用,可以作为FSHR32-44避孕疫苗的载体。

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