全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

雌马酚对去卵巢后大鼠骨质疏松症的影响

, PP. 256-260

Keywords: 去卵巢后骨质疏松症,雌马酚,雌激素水平

Full-Text   Cite this paper   Add to My Lib

Abstract:

目的观察雌马酚对去卵巢后大鼠骨质疏松症的作用。方法雌性SD大鼠去卵巢以建立骨质疏松症模型。健康3月龄雌性SD大鼠分为5组:假手术组(sham)、去卵巢组(ovarietomized,OVX)、OVX+雌马酚高剂量组[equol,Eq,0.5mg/(kg·d)]、OVX+雌马酚低剂量组[equol,Eq,?0.25mg?/(kg·d)]、OVX+雌二醇阳性对照组(estradiol,E2)。去卵巢8周后,按以上分组继续给药?8周?后取右侧股骨检测骨密度(bonemineraldensity,BMD),眼部取血检测血钙、血磷和骨钙素(boneglaprotein,BGP)含量;ELISA检测血清雌激素(estrogen,ES)、成骨细胞护骨素(osteoprotegerin,OPG),破骨细胞分化因子(receptoractivatorofnuclearfactorkappaBligand,RANKL)水平;qRT-PCR法检测OPG和RANKLmRNA的表达。结果OVX组与sham组比较,ES值、BMD和血钙水平显著下降(P<0.05),分别为sham组的78.1%、84.4%和83.2%。同时,OVX组大鼠血磷和BGP含量明显升高(P<0.05),表明去卵巢后骨质疏松症模型造模成功;OVX组大鼠与sham组比较,OPG蛋白和OPGmRNA表达量显著下降,RANKL蛋白和RANKLmRNA表达量明显升高(P<0.05),而Eq和E2组处理后能显著增加OPG蛋白和OPGmRNA表达,下调RANKL蛋白和RANKLmRNA表达,同时提高OVX大鼠的BMD和血钙水平,降低血磷和BGP含量(P<0.05)。结论雌马酚能有效改善去卵巢后大鼠骨质疏松症,可能与其能上调OPG而抑制RANKL分泌有关。

References

[1]  de-Matos O,Lopes-da-Silva D J,Martinez-de-Oliveira J, et al. Effect of specific exercise training on bone mineral density in women with postmenopausal osteopenia or osteoporosis[J]. Gynecol Endocrinol,2009,25(9):616-620.? [2]Al-Anazi A F,Qureshi V F,Javaid K, et al. Preventive effects of phytoestrogens against postmenopausal osteoporosis as compared to the available therapeutic choices:An overview[J]. J Nat Sci Biol Med,2011,2(2):154-163.? [3]Hoang-Kim A,Gelsomini L,Luciani D, et al. Fracture healing and drug therapies in osteoporosis[J]. Clin Cases Miner Bone Metab,2009,6(2):136-143.? [4]Cauley J A,Robbins J,Chen Z, et al. Effects of estrogen plus progestin on risk of fracture and bone mineral density:the Women’s Health Initiative randomized trial[J]. JAMA,2003,290(13):1729-1738.? [5]Vitale D C,Piazza C,Melilli B, et al. Isoflavones:estrogenic activity,biological effect and bioavailability[J]. Eur J Drug Metab Pharmacokine,2013,38(1):15-25. [6]Kolios L,Sehmisch S,Daub F, et al. Equol but not genistein improves early metaphyseal fracture healing in osteoporotic rats[J]. Planta Med,2009,75(5):459-465.? [7]Setchell K D,Brown N M,Summer S, et al. Dietary factors influence production of the soy isoflavone metabolite s-(-)equol in healthy adults[J]. J Nutr,2013,143(12):1950-1958.? [8]Dong J Y,Qin L Q. Soy isoflavones consumption and risk of breast cancer incidence or recurrence:a meta-analysis of prospective studies[J]. Breast Cancer Res Treat,2011,125(2):315-323.? [9]李菲,赵锦程,郭玉琴,等. 骨金散对去卵巢大鼠骨密度,雌激素水平的影响[J]. 黑龙江医药科学,2011,34(3):6-7. [10]Malairungsakul O,Wiwatanadate P. Factors related to osteoporosis of postmenopausal women in Phayao,Thailand[J]. Arch Osteoporos,2013,8(1/2):154.? [11]Levis S,Strickman-Stein N,Ganjei-Azar P, et al. Soy isoflavones in the prevention of menopausal bone loss and menopausal symptoms:a randomized,double-blind trial[J]. Arch Intern Med,2011,171(15):1363- 1369.? [12]Ma D F,Qin L Q,Wang P Y, et al. Soy isoflavone intake increases bone mineral density in the spine of menopausal women:meta-analysis of randomized controlled trials[J]. Clin Nutr,2008,27(1):57-64.? [13]Wang B,Mi M,Wang J, et al. Does the increase of endogenous steroid hormone levels also affect breast cancer risk in Chinese women? A case-control study in Chongqing,China[J]. Int J Cancer,2009,124(8):1892-1899.? [14]王建,徐静,王斌,等. 绝经后妇女膳食结构与骨质疏松症发生的相关性研究[J]. 第三军医大学学报,2011,33(22):2397-2401.? [15]Smith S Y,Jolette J,Turner C H. Skeletal health:primate model of postmenopausal osteoporosis[J]. Am J Primatol,2009,71(9):752-765.? [16]Corina M,Vulpoi C,Branisteanu D. Relationship between bone mineral density,weight,and estrogen levels in pre and postmenopausal women[J]. Rev Med Chir Soc Med Nat ?Iasi?,2011,116(4):946-950.? [17]谢煜,姚珍薇,杨帆. 雌激素和维生素 K 抗去势成年雌性大鼠骨质疏松的实验研究[J]. 中华妇幼临床医学杂志: 电子版,2009,5(3):226-231.? [18]Jabbar S,Drury J,Fordham J N, et al. Osteoprotegerin,RANKL and bone turnover in postmenopausal osteoporosis[J]. J Clin Pathol,2011,64(4):354-357.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133