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新型姜黄素纳米粒对Lewis肺癌细胞增殖、凋亡的影响

, PP. 141-145

Keywords: 姜黄素,纳米粒,Lewis肺癌,细胞增殖,凋亡

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Abstract:

目的探讨一种新型的姜黄素纳米粒(curcuminnanoparticles,Cur-NPs)对Lewis肺癌细胞LLC增殖与凋亡的影响。方法游离姜黄素(curcumin,Cur)和Cur-NPs分别作用于LLC细胞24h,MTT检测其对LLC细胞的增殖抑制作用。20μmol/L的药物作用于细胞24h后,流式细胞术检测Cur-NPs对细胞周期、细胞凋亡、细胞内活性氧、细胞内钙离子浓度、细胞线粒体膜电位的影响,并与Cur进行对照。结果Cur-NPs可以明显增强Cur对LLC细胞的增殖抑制,IC50从34.91μmol/L降为10.65μmol/L(P<0.05)。Cur-NPs明显提高了Cur诱导LLC细胞凋亡(P<0.05)、抑制细胞周期于S期(P<0.05)、升高细胞内钙离子浓度(P<0.05)、升高细胞活性氧浓度(P<0.05)、降低细胞线粒体膜电位(P<0.05)的能力。结论新型姜黄素纳米粒可明显提高游离姜黄素对LLC细胞的体外增殖抑制作用,可能与其促进细胞凋亡有关。

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