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沙眼衣原体隐匿质粒编码蛋白的表达及其相互作用

, PP. 34-38

Keywords: 沙眼衣原体,隐匿质粒,Pgp,蛋白相互作用

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Abstract:

目的分析沙眼衣原体隐匿质粒编码蛋白的相互作用。方法采用基因克隆的方法将沙眼衣原体质粒基因pgp1~4及pgp8分别克隆到pRAC和pRBR上,通过转录激活细菌双杂交系统分析蛋白相互作用。结果Westernblot结果显示Pgp1、Pgp3与大肠杆菌RpoA氨基末端功能区(α-NTD)融合蛋白及Pgp1、Pgp3、Pgp4与DNA结合蛋白λCI融合蛋白,能在大肠杆菌中表达。在报告菌株中,共表达与α-NTD融合的Pgp3蛋白(α-Pgp3)及与λCI融合的Pgp3蛋白(CI-Pgp3),导致报告基因产物β-半乳糖苷酶的活性升高。而余无明显变化。结论pgp3编码的Pgp3能与自身相互作用。

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