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不同16SrDNA靶序列PCR-DGGE分析油页岩细菌多样性

DOI: 10.3969/j.issn.1005-3026.2014.07.027, PP. 1033-1037

Keywords: 油页岩,16SrDNA,PCR-DGGE,细菌多样性,靶序列

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Abstract:

为全面了解油页岩细菌群落组成结构,同时筛选出适于油页岩PCR-DGGE的最佳引物,采用改进SDS高盐提取法,提取抚顺西露天矿油页岩微生物总DNA,并用968F/1401R,338F/518R,341F/907R和1055F/1406R,对16SrDNAV6-V8,V3,V8和V9区进行PCR-DGGE分析.结果表明,4组引物均能较好扩增出目的基因,但不同靶序列对多样性检出有显著影响(P<0001);与其他引物相比,968F/1401R和338F/518R获得的指纹图谱物种丰富度更高,检出细菌类群更丰富,较适合油页岩样品.油页岩细菌群落组成较简单,优势细菌包括不动杆菌属、假单胞菌属和埃希氏菌属,同时还有假单胞菌目和肠杆菌目尚未被鉴定的一些种类.

References

[1]  Bekoski V P,Mili J,Mandi B,et al.Removal of organically bound sulfur from oil shale by iron(III)ion generatedregenerated from pyrite by the action of Acidithiobacillus ferrooxidans—research on a model system[J].Hydrometallurgy,2008,94(1/2/3/4):8/13.
[2]  Haddadin M S Y,Arqoub A A A,Reesh I A,et al.Kinetics of hydrocarbon extraction from oil shale using biosurfactant producing bacteria[J].Energy Conversion and Management,2009,50(4):983/990.
[3]  Kim J S,Crowley D E.Microbial diversity in natural asphalts of the Rancho La Brea Tar Pits[J].Applied and Environmental Microbiology,2007,73(14):4579/4591.
[4]  Permanyer A,Gallego J L R,Caja M A,et al.Crude oil biodegradation and environmental factors at the Riutort oil shale mine,Se Pyrenees[J].Journal of Petroleum Geology,2010,33(2):123/140.
[5]  Matlakowska R,Sklodowska A.Biodegradation of kupferschiefer black shale organic matter (ForeSudetic Monocline,Poland) by indigenous microorganisms[J].Chemosphere,2011,83(9):1255/1261.
[6]  Watanabe K,Kodama Y,Harayama S.Design and evaluation of PCR primers to amplify bacterial 16S ribosomal DNA fragments used for community fingerprinting[J].Journal of Microbiological Methods,2001,44(3):253/262.
[7]  Brons J K,van Elsas J D.Analysis of bacterial communities in soil by use of denaturing gradient gel electrophoresis and clone libraries,as influenced by different reverse primers[J].Applied and Environmental Microbiology,2008,74(9):2717/2727.

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