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SpeciesidentificationrefinedbymolecularscatologyinacommunityofsympatriccarnivoresinXinjiang,China

, PP. 72-78

Keywords: DNAanalysis,Snowleopard,Scats,Noninvasivegenetics,Carnivore

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Abstract:

Manyecologicalstudiesandconservationmanagementplansemploynoninvasivescatsamplingbasedontheassumptionthatspecies'scatscanbecorrectlyidentifiedinthefield.However,inhabitatswithsympatricsimilarlysizedcarnivores,misidentificationofscatsisfrequentandcanleadtobiasinresearchresults.Toaddressthescatidentificationdilemma,molecularscatologytechniqueshavebeendevelopedtoextractDNAfromthedonorcellspresentontheouterliningofthescatsamples.Atotalof100sampleswerecollectedinthewinterof2009and2011inTaxkorganregionofXinjiang,China.DNAwasextractedsuccessfullyfrom88%ofsamplesandgeneticspeciesidentificationshowedthatmorethanhalfthescatsidentifiedinthefieldassnowleopard(Pantherauncia)actuallybelongedtofox(Vulpesvulpes).Correlationbetweenscatcharacteristicsandspecieswereinvestigated,showingthatdiameteranddryweightofthescatweresignificantlydifferentbetweenthespecies.Howeveritwasnotpossibletodefineapreciserangeofvaluesforeachspeciesbecauseofextensiveoverlapbetweenthemorphologicalvalues.Thispreliminarystudyconfirmsthatidentificationofsnowleopardfecesinthefieldismisleading.Researchthatreliesuponscatsamplestoassessdistributionordietofthesnowleopardshouldthereforeemploymolecularscatologytechniques.Thesemethodsarefinanciallyaccessibleandemployrelativelysimplelaboratoryproceduresthatcangiveanindisputableresponsetospeciesidentificationfromscats.

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