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大豆科学  2008 

大豆根际土壤镰孢菌不同分离方法比较

DOI: 10.11861/j.issn.1000-9841.2008.01.0106, PP. 106-112

Keywords: 大豆,根际土壤,镰孢菌,分离方法

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Abstract:

镰孢菌(Fusarium)引起的大豆根腐病是难防治的土传病害之一。土壤中镰孢菌多样性水平很高,并对其中致病菌种群动态及寄主抗性表现有一定影响。镰孢菌分离在其多样性研究中起重要作用,报道的镰孢菌分离方法很多,但分离效果有一定差异。本文对影响分离效果的相关内容进行了研究,比较了稀释平板法和土粒平板法。结果表明,当抗生素浓度增加到常量的4倍时可很好抑制细菌,并对真菌数目没有显著影响;不同抗生素组合对镰孢菌分离有一定影响;无菌水和水琼脂处理不同样品量与菌落数(CFU)之间均呈线性关系(R2>0.9),随着样品量的增加菌落数呈递增趋势;无菌水处理中镰孢菌的分离比率为21.0%,略高于水琼脂处理的10.0%;供试2种培养基对镰孢菌分离比率没有显著影响,与孔雀绿琼脂培养基(MGA)相比,蛋白胨-五氯硝基苯琼脂培养基(PPA)对镰孢菌的分离比率较高为20.93%。培养方法对镰孢菌分离比率影响较大,土粒平板法分离比率均大于60.0%,远高于稀释平板法(分离比率均低于6%)。

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