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大豆科学  2009 

大豆苗期固氮相关性状的QTL分析

DOI: 10.11861/j.issn.1000-9841.2009.04.0583

Keywords: 大豆,生物固氮,QTL

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Abstract:

大豆与根瘤菌共生固氮是大豆生长发育所需氮素的主要来源。由于根瘤菌与大豆两者基因型的不同,接种根瘤菌后大豆固氮能力也不同。以合丰25×固新野生大豆杂交组合的重组自交系(RIL)群体F11的104个株系为材料,在严格控菌条件下,用固氮菌株2178进行结瘤匹配鉴定,测定RIL群体及其亲本的固氮酶活性、结瘤数目、侧根数目、根瘤鲜重、茎干重5个指标,对所得数据进行正态分布检验,结合SSR分子数据利用复合区间作图法对其QTL定位分析。结果表明:RIL群体各性状均表现超亲分离,均值介于双亲之间,其偏度和峰度均较小,符合正态分布。这表明所考察性状均为数量性状遗传。应用复合区间作图法进行固氮性状的QTL定位,在A1、L、O、D1b、D2、C2、I连锁群,检测控制固氮的QTL有8个,解释表型变异的7.65%~15.05%,这些QTL及分子标记位点可用于大豆固氮性状的分子标记选择。

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