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大豆科学  2009 

大豆M型细胞质雄性不育恢复基因标记定位

DOI: 10.11861/j.issn.1000-9841.2009.04.0578, PP. 578-582

Keywords: 大豆,细胞质雄性不育,微卫星标记

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Abstract:

鉴于与恢复基因紧密连锁的分子标记在分子辅助选择育种中的应用前景,采用SSR标记法定位大豆CMS恢复系WR016的恢复基因。根据(W931A×WR016)F1、F2的植株育性,分析表明大豆M型不育系统为单基因配子体不育。由于大豆M-CMS恢复系WR016的恢复基因定位在A1连锁群上,利用A1连锁群上的大豆SSR引物对不育系W931A和恢复系WR016构建的F2分离群体进行分析,获得了与恢复基因连锁的三个标记Satt684、Satt276和Satt545,遗传距离分别为29.5cM、10.7cM和14.1cM。虽然10.7cM还是一个较远的距离,但为进一步精确定位恢复基因,并最终克隆恢复基因打下了基础。

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