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大豆科学  2009 

大豆分子标记遗传图谱的整合及其应用

DOI: 10.11861/j.issn.1000-9841.2009.04.0557

Keywords: 大豆遗传连锁图谱,整合图谱,SSR

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Abstract:

图谱整合是弥补单个作图群体因分子标记多态性的局限性而难以构建高密度图谱的有效方法。利用具明显农艺性状差异的大豆品种间杂交组合(科丰1号×南农1138-2、南农87-23×NG94-156、苏88-M21×新沂小黑豆和皖82-178×通山薄皮黄豆甲)所衍生的重组自交系群体分别构建了含有560,223,195,133个分子标记的遗传连锁图谱。以各图谱共有SSR标记作为锚定标记,使用JoinMap3.0进行图谱整合,得到一张包含20个连锁群,795个分子标记,总遗传距离2772.9cM,平均间距3.49cM的整合图谱。各连锁群的标记个数在24~69之间,遗传距离在77.1~224.7cM之间。与Song等的公共图谱比较,标记在连锁群上的分布和位置高度吻合,并增加了5个公共图谱上没有的SSR标记,另有6个SSR标记定位在不同的连锁群上。通过整合图谱可将关联分析所获基因/QTL定位到连锁群区间;便于不同群体定位结果间的比较;并找寻与之连锁更紧密的邻近标记。鉴于本图谱所用作图群体的亲本与国内育种常用材料的遗传来源相近,将更便于国内育种性状的QTL定位研究。

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