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大豆科学  2009 

不同光长条件下大豆蛋白质组比较研究

DOI: 10.11861/j.issn.1000-9841.2009.03.0388, PP. 388-393

Keywords: 大豆,双向电泳(-DE),蛋白质组学,光周期,离子阱质谱分析

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Abstract:

对大豆光周期敏感型品种东农42分别进行16h光照和8h光照处理,提取处理和对照相同叶位叶片的全蛋白质进行双向电泳,通过PDQuest和液相离子阱质谱(LC-ESI-MS)技术对差异表达蛋白进行分析,获得二级质谱图谱,用Mascot软件搜索NCBInr数据库,鉴定出16个差异表达蛋白,其中12个表达量上调,4个表达量下调。对谷胱甘肽S??转移酶、二硫键异构酶、TCTP、半胱氨酸合成酶、Rieske铁硫蛋白前体、金属蛋白酶等主要差异蛋白进行功能分析,表明这些蛋白与植物的光合作用、信号转导、细胞凋亡的调控、防卫解毒和能量代谢等多种植物的生理生化功能相关。

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