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大豆科学  2009 

大豆异黄酮甙元对结肠癌HT-29细胞增殖和凋亡的影响

DOI: 10.11861/j.issn.1000-9841.2009.02.0310, PP. 310-313

Keywords: 大豆,异黄酮,甙元,结肠癌,细胞凋亡

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Abstract:

大豆异黄酮通过促进肿瘤细胞凋亡可抑制多种肿瘤细胞的增殖。从大豆提取大豆异黄酮糖甙,再将其部分水解成其甙元,研究大豆异黄酮甙元抑制结肠癌细胞的增殖和促进其凋亡的作用。采用MTT比色法观察大豆异黄酮甙元对结肠癌HT-29细胞增殖的影响,采用TUNEL染色法检测其对HT-29细胞凋亡的影响,采用免疫细胞化学法检测凋亡相关蛋白bax、bc1-2和p53的表达。结果表明:大豆异黄酮甙元可在20~80mgL-1范围内时间和浓度依赖性地抑制结肠癌HT-29细胞增殖和诱导细胞凋亡。用40mgL-1大豆异黄酮甙元作用结肠癌HT-29细胞72h时,细胞生长抑制率为(57.1±4.9)%,其对肿瘤细胞凋亡率为(20.9±2.1)%。免疫组化结果还显示,大豆异黄酮甙元可显著性增加HT-29细胞凋亡相关基因bax蛋白表达和降低bc1-2表达。提示,大豆异黄酮甙元可通过诱导结肠癌细胞凋亡发挥抗结肠癌作用。

References

[1]  周建芹.大豆异黄酮提取工艺优化及其活性研究[J].大豆科学,2007,26(2):276-279.(Zhou J Q.Optimization of extraction technology of soybean isoflavones and its physiological activity analysis[J].Soybean Science,2007,26(2):276-279.)
[2]  Wang H J,Murphy P A.Isoflavone contents in commercial soybean foods[J].Journal of Agricultural and Food Chemistry,1994,42(8):1666-1673.
[3]  Vedavanam K,Srijayanta S,O’Reilly J,et al.Antioxidant action and potential antidiabetic properties of an isoflavonoid-containing soybean phytochemical extract(SPE)[J].Phytotherapy Research,1999,13(7):601-608.
[4]  Kerry N,Abbey M.The isoflavone genistein inhibits copper and peroxyl radical mediated low density lipoprotein oxidation in vitro[J].Atherosclerosis,1998,140(2):341-347.
[5]  Tophkhane C,Yang S,Bales W,et al.Bcl-2 overexpression sensitizes MCF-7 cells to genistein by multiple mechanisms[J].International Journal of Oncology,2007,31(4):867-874.
[6]  Chodon D,Ramamurty N,Sakthisekaran D.Preliminary studies on induction of apoptosis by genistein on HepG2 cell line[J].Toxicology in vitro :an international journal published in association with BIBRA,2007,21(5):887-891.
[7]  Dewell A,Weidner G,Sumner M D,et al.Relationship of dietary protein and soy isoflavones to serum IGF-1 and IGF binding proteins in the prostate cancer lifestyle trial[J].Nutrition and cancer,2007,58(1):35-42.
[8]  全吉淑,沈明花,柳明洙,等.大豆异黄酮苷元的提取及其抗血浆脂蛋白氧化作用[J].食品科技,2008,33(1):126-128.(Quan J S,Shen M H,Liu M Z,et al.Isolation of soy isoflavone aglycones and inhibition on oxidation of plasma lipoproteins[J].Food Science and Technology,2008,33(1):126-128.)
[9]  Melet A,Song K,Bucur O,et al.Apoptotic pathways in tumor progression and therapy[J].Advances in Experimental Medicine and Biology,2008,615:47-79.
[10]  Tan T T,White E.Therapeutic targeting of death pathways in cancer:mechanisms for activating cell death in cancer cells[J].Advances in Experimental Medicine and Biology,2008,615:81-104.
[11]  Lu Q L,Poulsom R,Wong L,et al.Bcl-2 expression in adult and embryonic non-haematopoietic tissues[J].The Journal of Pathology,1993,169(4):431-437.
[12]  Korsmeyer S J.Bcl-2 gene family and regulation of programmed cell death[J].Cancer Research,1999,59(7s):1693-1700.
[13]  Song G,Mao Y B,Cai Q F,et al.Curcumin induces human HT-29 colon adenocarcinoma cell apoptosis by activating p53 and regulating apoptosis-related protein expression[J].Brazilian zjournall of Medical and Biological Research,2005,38(12):1791-1798.

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