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大豆科学  2009 

抗草甘膦转基因大豆(RRS)在黑土生态系统种植的安全性研究

DOI: 10.11861/j.issn.1000-9841.2009.02.0260, PP. 260-265

Keywords: 抗草甘膦基因,转基因大豆,黑土生态系统,土壤细菌多样性

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Abstract:

为明确抗草甘膦转基因大豆(RRS)在黑土生态系统种植的安全性,经过连续3年田间和盆栽试验,分析了抗草甘膦转基因漂移(漂流)可能性,及抗草甘膦转基因大豆(RRS)在黑土区对根际生态系统微生物数量,氮代谢和两种土壤酶活性的影响。结果表明:在自然条件下花粉漂移几乎是不可能的,但如人为加大虫媒传播(大于10头m-2),抗草甘膦基因的漂移概率接近0.05%,漂移距离为0.7m。RRS除对根际真菌数影响不显著外,对根际土壤细菌、放线菌、氨化和硝化细菌均表现降低趋势。在大豆不同生育期,RRS除对根际土壤真菌数影响不显著外,对根际土壤细菌,放线菌,氨化和硝化细菌均表现降低趋势;RRS根际土壤细菌多样性指数与均匀度指数均低于亲本RRS-S;氨化强度与RRS-S相比差异不显著,RRS根际土壤硝化强度显著低于亲本RRS-S;RRS降低了过氧化氢酶和脲酶活性。

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