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大豆科学  2009 

紫外线B辐射增强对大豆生长及光合作用相关指标的影响

DOI: 10.11861/j.issn.1000-9841.2009.02.0229

Keywords: UV??B,大豆,光合作用

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Abstract:

明确UV-B辐射增强对植物生长发育,生物量以及光合作用相关指标的影响,有助于阐明UV-B辐射对植物影响的生理机制。在人工气候室内采用平方波模型,在大豆植株上方悬挂紫外灯,照射强度为15μWcm-2,每天照射8h。研究在此剂量下大豆植株在干物质积累、色素含量、可溶性蛋白质含量、PSⅡ反应中心最大光能转换效率以及光合速率的变化。结果表明:UV-B辐射增强对大豆生长具抑制作用。与对照相比,播种后20d、40d和60d的UV-B处理的大豆植株株高分别降低4.9%、13.8%和10.6%;鲜重及干重分别减少17.8%、10.7%、25.5%及22.3%、20.9%、25.6%;叶绿素含量分别降低3.2%、13.2%和29.4%;PSⅡ反应中心最大光能转换效率(Fv/Fm)分别降低3.4%、1.3%和3.2%;叶片可溶性蛋白质含量分别降低25.3%、45.2%和19.5%;叶面积分别减小15.2%、20.4%、和41.6%;光合速率分别降低10%、25%和16%。作为一种保护性适应,UV-B处理增加了花青素及类胡萝卜素的含量。UV-B辐射对生长和生物量的抑制作用可能主要是由于光合速率下降及有效光合面积减少所致。

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