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大豆科学  2009 

子房滴注法将GUS基因表达框转入大豆的研究

DOI: 10.11861/j.issn.1000-9841.2009.02.0191, PP. 191-194

Keywords: 大豆,无载体,无选择标记,GUS基因表达框,子房滴注,转化

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Abstract:

为了验证大豆子房滴注转化方法的可重复性与遗传稳定性,将由报告基因GUS、表达调控序列(35S启动子,NOS终止子)和T-DNA边界序列3部分组成的基因表达框转入大豆。对T0代转化材料进行GUS组织化学染色分析和PCR检测。结果表明:在检测的340个幼胚中有12个呈GUS阳性,且在180个转化植株中有6个呈PCR阳性同时其叶片也呈GUS阳性,转化率分别为3.53%和3.33%。Southern杂交表明外源GUS基因表达框以低拷贝的形式整合到大豆基因组中。对转基因后代叶片GUS染色、PCR分析及Northernblot检测结果表明外源基因已遗传给了后代,其中S2株系符合孟德尔分离规律。

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