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大豆科学  2009 

乙烯、6-BA对大豆幼苗生长、生化成分及细胞组织结构的效应

DOI: 10.11861/j.issn.1000-9841.2009.01.0041

Keywords: 大豆幼苗,ETH,-BA,形态特征,生化成份,细胞组织结构

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Abstract:

采用人工培养箱技术,研究ETH、6-BA及其复配处理对大豆幼苗的形态特征、生物量、生化成分、内源激素以及细胞组织结构的调节效应,为大豆芽菜的安全生产提供理论和技术支持。结果表明:不同处理之间大豆幼苗形态存在差异(P<0.05)。ETH、6-BA及复配处理,均抑制了大豆幼苗的根和下胚轴的生长,促进下胚轴的增粗,三者处理后的根长分别比对照减少了24.1%(P<0.01),44.7%(P<0.01)和51.7%(P<0.01);下胚轴长分别比对照减少39.2%(P<0.01),4.1%(P>0.05)和26.2%(P<0.05);下胚轴粗分别比对照增加18.5%(P<0.01),9.2%(P<0.05)和32.3%(P<0.01)。大豆幼苗形态上的差异可以通过其下胚轴细胞组织结构的变化来解释,ETH促进了细胞的横向分裂与皮层细胞的扩大,促进下胚轴的增粗,抑制了细胞的径向分裂;6-BA促进了皮层细胞的横向分裂与扩大,抑制了细胞径向的伸长;ETH-6-BA复配处理促进了皮层薄壁细胞的分裂与扩大。不同处理之间大豆幼苗的维生素C含量存在差异(P<0.05);与对照相比,处理组大豆幼苗的GA含量降低,ABA含量增高(P<0.01)。

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