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大豆科学  2009 

大豆抗旱种质资源遗传多样性的RAPD分析

DOI: 10.11861/j.issn.1000-9841.2009.01.0028

Keywords: 大豆,遗传多样性,RAPD,聚类分析

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Abstract:

采用RAPD技术对45个不同抗旱程度的大豆种质进行遗传多样性分析。从115条随机引物筛选出10条重复性好、扩增条带清晰的引物,然后对45个品种基因组DNA进行扩增,结果共扩增出86条片段,其中同源片段17条,占带总数的19.8%,多态性片段69条,占片段总数的80.2%,平均每条引物扩增出多态性片段6.9条,大部分片段介于200~2000bp之间。利用NTSYS-Pc软件计算品种间遗传相似系数(GS)和遗传距离(GD),45个品种之间的遗传相似系数介于0.3818~0.9038之间。在GD=0.38处,将45份抗旱性不同的大豆品种分成3大类,第一类有40个品种,占品种总数的90%,第二类包含2个品种,分别是低抗旱的6178和不抗旱的大白眉,第三类有3个品种,分别是不抗旱的Fayette、牛毛黄和90-1105。在GD=0.29处,第一大类群又按照抗旱性的高低分别聚成高抗旱、中抗旱、低抗旱、不抗旱等10个亚类群。聚类结果反映出品种间关系与地理起源、表型形态等具有一定的相关性。

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