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大豆科学  2009 

大豆异黄酮生物合成串联基因簇及其表达载体的构建

DOI: 10.11861/j.issn.1000-9841.2009.01.0007

Keywords: 大豆异黄酮,生物合成,串联基因簇,表达载体

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Abstract:

植物异黄酮是一种重要的次级代谢产物,在植物的生理活动以及人类健康方面具有重要作用。植物异黄酮生物合成途径有三个调控酶:查尔酮合酶(Chalconesynthase,CHS),查尔酮异构酶(ChalconeIsomerase,CHI)和异黄酮合酶(Isoflavonesynthase,IFS)。以大豆为材料,利用RT-PCR方法克隆到查尔酮合酶基因(CHS),查尔酮异构酶基因(CHIⅡ)和异黄酮合酶基因(IFS)的cDNA(Genebank登记号分别为EU526827,EU526829,EU526830)经过NCBI在线Blast比对,相似度均在98%以上,确认克隆的目标基因是正确的;将这三种基因顺序连接,构建成串联基因簇CHS-CHIⅡIFS(命名为rIFS),并且构建了rIFS的原核表达载体,进行了初步的表达研究。为进一步研究植物异黄酮的生物合成以及进行异黄酮生物工程奠定了基础。

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