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大豆科学  2007 

大豆花粉管通道法转化lkt50基因

DOI: 10.3969/j.issn.1000-9841.2007.05.003, PP. 653-656

Keywords: 大豆,花粉管通道法,lkt

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Abstract:

采用花粉管通道法,将外源基因lkt50向大豆品种东农48和品系东农96-02导入。通过卡那霉素筛选,从所获得的种子苗中初选转化植株,进一步进行PCR、Southernblot及RT-PCR分子生物学检测。结果表明:在14株卡那霉素抗性材料中利用nptII基因的特异性引物有7株PCR阳性,利用lkt-50基因特异性引物有2株PCR阳性,并且2株Southernblot及RT-PCR检测均呈现阳性,因此认为花粉管通道法可以用于大豆的转基因研究和应用。

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