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大豆科学  2007 

大豆菌核病鉴定方法比较及分析

DOI: 10.3969/j.issn.1000-9841.2007.05.018, PP. 728-731

Keywords: 大豆菌核病,鉴定方法,比较,分析

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Abstract:

大豆菌核病鉴定方法对于大豆菌核病的研究至关重要,本文比较了子叶接种、离体茎段接种、离体叶片接种和离体茎的草酸反应4种大豆菌核病鉴定方法。结果表明:利用子叶接种法接种5d后所有植株的子叶全部干枯皱缩,10d后敏感品种合丰25植株开始萎蔫变黄,接种部位周围开始出现白色菌丝;15d后植株布满白色菌丝;20d后发病植株开始死亡;而耐性品种MAPLEARROW出现这些症状的时期较晚,死亡率也较低。离体茎段接种法和离体叶片接种法,基本上体现了大豆菌核病的主要致病特征。在离体茎的草酸接种中,合丰25和MAPLEARROW对草酸的敏感度明显不同,且受害程度有明显差别,是一种方便快捷而且准确性较高的鉴定方法,对于大量种质资源筛选有着重要意义。

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