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大豆科学  2008 

钙对铜胁迫下大豆种子的解毒作用

DOI: 10.11861/j.issn.1000-9841.2008.06.1072, PP. 1072-1075

Keywords: 铜胁迫,,大豆,保护酶

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Abstract:

钙对铜胁迫下大豆种子解毒作用的研究,对获得优良抗逆性状和提高大豆产量具有重要的意义。设置0、0.4、0.8、2.4和4mmol.L-1铜离子浓度梯度,同时在各浓度梯度中分别添加4.5和9.0mmol.L-1的钙离子,进行了种子萌发试验,测定了相对电导率和保护酶活性。结果显示:铜对大豆种子的发芽活性具有抑制作用,且随胁迫浓度的增加这种抑制作用加强,钙离子对0.8mmol.L-1铜离子的解毒作用效果明显,能够提高种子的活力指数和根长,减少电解质渗透率。4.5mmol.L-1钙能够增加铜胁迫下大豆种子中POD活性,而降低CAT活性,两种酶存在着一种协调关系。说明钙对一定浓度的铜胁迫能够起到缓解作用,其中保护酶参与了细胞膜完整性的维持。

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