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大豆科学  2008 

一株高产谷氨酰胺酶菌株的鉴定和酶活特性的研究

DOI: 10.11861/j.issn.1000-9841.2008.06.1037, PP. 1037-1040

Keywords: 谷氨酰胺酶,酱油,柠檬酸细菌,鉴定

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Abstract:

对筛选得到一株高产谷氨酰胺酶菌株进行了初步鉴定并对酶活特性做了研究。通过生理生化实验并结合菌体形态特性,结果发现该菌株可以确定该菌属于柠檬酸细菌属(Citrobacter)。通过单因素试验,考察了温度、pH、NaCl和产物谷氨酸对该菌株产生的谷氨酰胺酶的影响。结果发现菌株产生的谷氨酰胺酶的最佳反应pH为6.2,酶最适反应温度为50℃。酶最稳定的pH为6.2,温度低于40℃,酶具有较高的稳定性。高浓度的NaCl对谷氨酰胺酶活性基本无影响,谷氨酸对酶也没有明显的抑制作用。

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