全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
大豆科学  2008 

一种野生大豆根际土壤细菌的分离及其防治大豆病害的研究

DOI: 10.11861/j.issn.1000-9841.2008.06.1007

Keywords: 野生大豆,拮抗,大豆连作

Full-Text   Cite this paper   Add to My Lib

Abstract:

为筛选拮抗大豆根腐病的土壤微生物,以邻苯二甲酸为唯一碳源培养基,从野生大豆根际土壤中分离获得1株细菌,命名为HK26-21。通过平皿接种以及16srDNA序列扩增,根据其生理生化特征和16srDNA(GenBankAccessionNo.EF032879)序列相似性分析,将该菌株鉴定为芽孢杆菌属(Bacillus)。通过单因素试验法确定了最佳降解条件:温度为25℃,pH为7.0,降解速率与接种量呈正相关。平皿微生物对峙试验表明菌株可拮抗大豆菌尖孢镰刀菌和立枯丝核菌。初步认为所分离野生大豆根际芽孢杆菌HK26-21可以缓解大豆连作障碍。

References

[1]  刘佩印.黑龙江省大豆重迎茬问题的研究概况[J].黑龙江农业科学,2001(3):60-64.(Liu P Y.A survey of continuous and every other cropping of soybean in Heilongjiang province[J].Heilongjiang Agricultural Sciences,2001(3):60-64.)
[2]  徐凤花,汤树德,孙冬梅,等.重迎茬对大豆根际微生物的影响[J].黑龙江八一农垦大学学报,1998,10(1):5-8.(Xue F H,Tang S D,Sun D M,et al.The effect of continous and one year intermittent cropping on rhizosphere microbe of soybean[J].Journal of Heilongjiang August First Land Reclamation University,1998,10(1):5-8.)
[3]  何志鸿,刘忠堂,许艳丽,等.大豆重迎茬减产的原因及农艺对策研究―重迎茬大豆的根际土壤有机化合物[J].黑龙江农业科学,2003(5):1-5.(He Z H,Liu Z T,Xu Y L,et al.Study on the reason reducing production of soybeans planted continuously and the way to get more output-organic compound of rhizosphere soil[J].Heilongjiang Agricultural Sciences,2003(5):1-5.)
[4]  王树起,韩丽梅,杨振明.丙二酸和邻苯二甲酸对大豆生长发育的化感效应[J].吉林农业科学,2001,26(5):15-19.(Wang S Q,Han L M,Yang Z M.Allelopathy of 1,2-benzennedicarboxylic acid and malonic acid on soybean growth [J].Jilin Agricultural Sciences,2001,26(5):15-19.)
[5]  谌晓曦,陈卫良,李德葆.357细菌及其拮抗物质对植物病原真菌的抑制[J].微生物学通报,2003,30(3):67-71.(Chen X X,Chen W L,Li D B.The antagonistic activities of both bacillus cereus 357 and its antagonistic substance against botytis cinerea pers.and rhizoctonia solani[J].Microbiology,2003,30(3):67-71.)
[6]  胡春江,薛德林,王书锦,等.大豆连作障碍研究Ⅲ海洋放线菌MB-97促进大豆连作增产机理[J].应用生态学报,2002,13(9):1095-1098.(Hu C J,Xue D L,Wang S J,et al.Obstacles of soybean continuous cropping Ⅲ.Mechanism of soybean yield increment by marine actinomyces MB-97[J].Chinese Journal of Applied Ecology,2002,13(9):1095-1098.)
[7]  郭荣君,刘杏忠,杨怀文,等.芽孢杆菌BH1防治大豆根腐病的效果及机制[J].中国生物防治,2003,19(4):180-184.(Guo R J,Liu X Z,Yang H W,et al.Mechanism of rhizobacteria BH1(Bacillus sp.)to suppress soybean root rot disease caused by fusarium spp[J].Chinese Journal of Biological Control,2003,19(4):180-184.)
[8]  温广月,许艳丽,李春杰,等.6株生防细菌对大豆根腐病防治效果初步评价[J].大豆科学,2005,24(2):121-125.(Wen G Y,Xu Y L,Li C J,et al.Evaluation of six potential biocontrol against soybean root rot[J].Soybean Science,2005,24(2):121-125.)
[9]  李淑彬,刘玉焕,刘芳,等.降解甲胺磷农药高效菌株的筛选[J].湘潭师范学院学报,1998,19(3):60-64.(Li S B,Liu Y H,Liu F,et al.Screening of highly efficien bacteria of degrading methamidophos-pesticide[J].Journal of Xiangtan Normal University,1998,19(3):60-64.)
[10]  张忠辉,洪青,张国顺,等.杀螟硫磷降解菌 FDS-1的分离鉴定及其降解特性[J].中国环境科学,2005,25(1):52-56.(Zhang Z H,Hong Q,Zhang G S,et al.Identification and characterization of FDS-1[J].China Environmental Science,2005,25(1):52-56.)
[11]  李文鹏,吴颖运,张克勤.一种简便的胨冻样芽孢杆菌(Bacillus mucilaginosus)定向驯育及保藏方法[J].土壤通报,2003,34(6):602-604.(Li W P.Wu Y Y,Zhang K Q.A simple and convenient method for directive breeding and preservation of ?Bacillus mucilaginosus[J].Chinese Journal of Soil Science,2003,34(6):602-604.)

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133